节拍剂量卡培他滨对大鼠肝再生的影响  

Experimental observation of the effect of metronomic capecitabine on liver regeneration in rats

作　　者：杨蕊宁 王昊 孔德俊 孙茜 王政禄[3] 曹磊[4] 任家书 陈涛 段金良 卢嘉宁 李姗霓[3] 郑虹[3,4] Yang Ruining;Wang Hao;Kong Dejun;Sun Qian;Wang Zhenglu;Cao Lei;Ren Jiashu;Chen Tao;Duan Jinliang;Lu Jianing;Li Shanni;Zheng Hong(The First Central Clinical School,Tianjin Medical University,Tianjin 300190,China;?School of Medicine,Nankai University,Tianjin 300074,China;Organ Transplant Department,Tianjin First Central Hospital,School of Medicine,Nankai University,Tianjin 300192,China;Research Institute of Transplant Medicine,Nankai University,Tianjin 300070,China)

机构地区：[1]天津医科大学一中心临床学院,天津300190 [2]南开大学医学院,天津300074 [3]天津市第一中心医院器官移植科,天津300192 [4]南开大学移植医学研究院,天津300070

出　　处：《中华实验外科杂志》2024年第10期2195-2200,共6页Chinese Journal of Experimental Surgery

基　　金：国家重点研发计划项目(2020YFA0710802);天津市自然科学基金面上项目(22JCYBJC01230);天津市自然科学基金重点项目(21JCZRJC00160);天津市卫生健康委员会科学基金(ZC20065,ZC20089)。

摘　　要：目的:观察节拍剂量卡培他滨对70%肝切除大鼠肝再生的影响。方法:研究选取雄性Wistar大鼠,采用随机数字表法分为3组,每组18只:治疗组接受节拍剂量卡培他滨处理,溶剂组接受同等体积1%羧甲基纤维素钠溶剂处理,对照组仅手术不做其余处理。3组大鼠中分别进行70%肝切除术。术后3、5、7 d每组分别处死6只大鼠,通过肝脏再生率、组织病理学和分子生物学评估肝再生情况。两组间均数比较采用独立样本 t检验,3组间比较采用方差分析。 结果:节拍剂量卡培他滨不影响大鼠术后肝再生率,3、5、7 d治疗组与对照组及溶剂组比较差异无统计学意义[(82.90±3.78)%比(79.12±5.26)%、(82.36±6.38)%, t=0.719、0.085, P>0.05;(89.10±1.63)%比(87.46±4.65)%、(92.74±4.75)%, t=0.351、0.767, P>0.05;(116.5±1.15)%比(115.4±5.40)%、(116.5±5.32)%, t=0.212、0.005, P>0.05];节拍剂量卡培他滨不影响大鼠肝功能,术后3、5、7 d治疗组、对照组和溶剂组之间的血清肝功能指标(谷丙转氨酶、谷草转氨酶、白蛋白、碱性磷酸酶、γ-谷氨酰基转移酶、总胆红素)差异均无统计学意义[(24.18±6.10) U/L比(30.28±4.14) U/L比(33.08±4.06) U/L、(63.10±27.13) U/L比(90.23±16.78) U/L比(77.83±17.17) U/L、(11.13±0.23) g/L比(11.35±0.25) g/L比(11.60±0.24) g/L、(241.10±9.20) U/L比(250.30±19.07) U/L比(219.60±27.87) U/L、(1.53±0.13) U/L比(1.40±0.31) U/L比(1.88±0.44) U/L、(1.29±0.37) μmol/L比(1.66±0.22) μmol/L比(1.59±0.21) μmol/L, F=2.097、2.123、1.398、0.714、0.875、1.515, P>0.05;(22.18±1.45) U/L比(23.63±2.43) U/L比(22.35±2.31) U/L、(47.97±5.11) U/L比(53.08±9.45) U/L比(50.40±7.46) U/L、(17.73±1.13) g/L比(18.85±0.55) g/L比(18.08±0.40) g/L、(120.40±29.15) U/L比(149.50±20.99) U/L比(115.40±18.76) U/L、(1.15±0.03) U/L比(1.13±0.27) U/L比(1.05±0.31) U/L、(1.21±0.13) μmol/L比(1.34±0.23) μmol/L比(0.82±0.24) μmol/L, F=0.183、3.120、0.196、2.187、0.076、3.444, P>0.05;Objective:The impact of studying the metronomic capecitabine on liver regeneration in rats undergoing 70%hepatectomy.Methods:The male Wistar rats were randomly divided into three groups,with 18 rats in each group:The treatment group received metronomic capecitabine,the solvent group received an equal volume of 1%carboxymethyl cellulose sodium orally,and the control group underwent surgery without further treatment.All three groups of rats underwent a 70%hepatectomy.Six rats from each group were euthanized on postoperative days 3,5,and 7,and liver regeneration was assessed through parameters such as liver regeneration rate,histopathology,and molecular biology.The comparison of means between two groups uses independent samples t-test,while comparison among three groups uses analysis of variance(ANOVA).Results:The results showed that the metronomic capecitabine in the rhythm group did not affect the rat liver regeneration rates at 3,5,and 7 days after surgery.There were no statistically significant differences between the treatment groups compared to the control and solvent groups[(82.90±3.78)%vs.(79.12±5.26)%,(82.36±6.38)%,t=0.719,0.085,P>0.05;(89.10±1.63)%vs.(87.46±4.65)%,(92.74±4.75)%,t=0.351,0.767,P>0.05;(116.5±1.15)%vs.(115.4±5.40)%,(116.5±5.32)%,t=0.212,0.005,P>0.05].There were no statistically significant differences in serum liver function indicators(alanine aminotransferase,aspartate aminotransferase,albumin,alkaline phosphatase,gamma-glutamyl transferase,total bilirubin)among the treatment groups(3d,5d,7d post-operation),control group,and solvent group[(24.18±6.10)U/L vs.(30.28±4.14)U/L vs.(33.08±4.06)U/L,(63.10±27.13)U/L vs.(90.23±16.78)U/L vs.(77.83±17.17)U/L,(11.13±0.23)g/L vs.(11.35±0.25)g/L vs.(11.60±0.24)g/L,(241.10±9.20)U/L vs.(250.30±19.07)U/L vs.(219.60±27.87)U/L,(1.53±0.13)U/L vs.(1.40±0.31)U/L vs.(1.88±0.44)U/L,(1.29±0.37)μmol/L vs.(1.66±0.22)μmol/L vs.(1.59±0.21)μmol/L,F=2.097,2.123,1.398,0.714,0.875,1.515,P>0.05;(22.18±1.45)U/L vs.(23.63±2.43)U/L vs.(22.35±2.31

关 键 词：肝再生 卡培他滨 肝切除 

分 类 号：R657.3[医药卫生—外科学] R-332[医药卫生—临床医学]