多巴胺配位银离子共交联的脱细胞猪心包力学及生物学特性  

Mechanical and biological characterization of decellularized porcine pericardium co-crosslinked with dopamine-liganded silver ions

作　　者：张玉卿 陈星 赵金平[1] 刘金平 周建良 Zhang Yuqing;Chen Xing;Zhao Jinping;Liu Jinping;Zhou Jianliang(Department of Cardiovascular Surgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院心血管外科,武汉430071

出　　处：《中华实验外科杂志》2024年第10期2274-2276,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(82270382)。

摘　　要：目的:多巴胺、银离子共交联脱细胞猪心包(DA/Ag-PP)力学及生物相容性研究。方法:采用0.2 g/ml的多巴胺和12μg/ml银离子共同交联脱细胞的猪心包,并对比交联后的支架与原始脱细胞猪心包和戊二醛交联的猪心包之间的力学性能、生物学相容性等。通过单轴拉伸实验、电镜扫描、水接触角测量、细胞毒性实验、皮下包埋等实验,从各个角度综合评估多巴胺和银离子共交联的猪心包的各项性能,采用单因素方差分析。结果:单轴拉伸实验显示共交联后拉伸强度DA/Ag-PP组[(13.560±1.539)MPa,P<0.01]显著高于DPP组[(2.301±0.588)MPa],稍高于GPP组[(10.490±0.640)MPa,P<0.05]。此外,DA/Ag-PP组[(31.230±5.316)MPa]杨氏模量较DPP组[(8.173±1.095)MPa,P<0.01],但稍低于GPP组[(43.100±9.161)MPa,P>0.05]。扫描显微镜和院子里显微镜扫描显示DA/Ag-PP表面更粗糙。水接触角实验提示DA/Ag-PP[(46.230±0.153)°]水接触角较DPP组[(60.400±5.478)°,P<0.01]更小,但与GPP组[(48.470±1.159)°,P>0.05]差异无统计学意义。Raw264.7和HUVECs细胞毒性实验均显示DA/Ag-PP细胞毒性较DPP组稍差,但仍较戊二醛修饰组具有良好的生物安全性。在大鼠皮下包埋模型中DA/Ag-PP组表现出优异的再细胞化能力。结论:多巴胺和银离子共交联的脱细胞猪心包具有良好的力学性能和生物相容性。支架皮下包埋后,再细胞化水平较高,具有良好的重塑基础。Objective:Mechanical property and biocompatibility of dopamine and silver ions co-crosslinked decellularised porcine pericardium(DA/Ag-PP).Methods:The decellularized porcine pericardium was co-crosslinked with 0.2 g/ml of dopamine and 12μg/ml of silver ions,and the mechanical properties and biocompatibility of the crosslinked scaffolds(DA/Ag-PP)were compared with those of the original decellularized porcine pericardium(DPP)and glutaraldehyde-crosslinked porcine pericardium(GPP).The various properties of porcine pericardium co-crosslinked with dopamine and silver ions were comprehensively evaluated from various angles by uniaxial tensile experiments,electron microscope scanning,water contact angle measurements,cytotoxicity experiments,and subcutaneous embedding.Data are expressed as mean±standard deviation(Mean±SD)and analysed by one-way ANOVA Results:Uniaxial tensile experiments showed that DA/Ag-PP exhibited higher tensile strength[DPP:(2.301±0.588)MPa;DA/Ag-PP:(13.560±1.539)MPa;GPP:(10.490±0.640)MPa]and elastic modulus[DPP:(8.173±1.095)MPa;DA/Ag-PP:(31.230±5.316)MPa;GPP:(43.100±9.161)MPa].Scanning microscope and yard microscope scans showed greater surface roughness of DA/Ag-PP.Water contact angle experiments suggested that DA/Ag-PP water contact angle was smaller[DPP:(60.400±5.478)°;DA/Ag-PP:(46.230±0.153)°;GPP:(48.470±1.159)°].Raw264.7 and HUVECs cytotoxicity experiments both showed that DA/Ag-PP cytotoxicity was slightly worse than that of decellularized porcine pericardium alone,but still had excellent biosafety compared with the glutaraldehyde-modified group.In the rat subcutaneous embedding model,the DA/Ag-PP group showed excellent recellularization ability and certain biodegradability.Conclusion:The decellularized porcine pericardium co-crosslinked with dopamine and silver ions presented excellent mechanical properties and biocompatibility but was limited to the low safe concentration of silver ions,and within the cell-safe concentration,it is not yet able to exert significant metal immuno

关 键 词：脱细胞猪心包 瓣膜改性 多巴胺 金属免疫治疗 

分 类 号：R318.08[医药卫生—生物医学工程] R-332[医药卫生—基础医学]