铁皮石斛多糖提取 鉴定及其修护功效研究  

Research on the Extraction,Identification and Repair Efficacy of Polysaccharides from Dendrobium officinale

作　　者：陈冬球 杜冰[1] CHEN Dongqiu;DU Bing(College of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《农产品加工》2024年第22期91-95,共5页Farm Products Processing

摘　　要：选用新鲜铁皮石斛茎条为原料,通过水提醇沉法得到铁皮石斛多糖粉末样品,用高效凝胶渗透色谱法测试其多糖分子量,用离子色谱法测定其单糖组成,并研究的铁皮石斛多糖用细胞划痕愈合试验验证其修护功效。结果表明,水提醇沉法从铁皮石斛鲜茎条中提取铁皮石斛多糖的提取率约为23.45%,其分子量(Mw)约为410000 Da,占比为大于94%,其余分子量约为3500 Da,其中甘露糖含量约为66%,葡萄糖含量约为12.87%,且可明显促进细胞划痕愈合率,预示其具有优异的修护功效。The powder samples of Dendrobium officinale polysaccharide were obtained from fresh Dendrobium officinale stems by water extraction and ethanol precipitation.The molecular weight of the polysaccharide were tested by high performance gel permeation chromatography,and the monosaccharide composition was determined by ion chromatography.The results showed that the extraction rate of polysaccharides from fresh stems of Dendrobium officinale by water extraction and alcohol precipitation method was about 23.45%,with a molecular weight(Mw)of about 410000 Da accounting for more than 94%,and the remaining molecular weights of about 3500 Da.Among them,the content of mannose was about 66%,and the content of glucose was about 12.87%.It could significantly promote the healing rate of cell scratches,indicating that it has excellent repair effects.

关 键 词：铁皮石斛 多糖 甘露糖 修护 

分 类 号：TQ658.2[化学工程—精细化工]