小麦CSase基因家族的鉴定及表达分析  

Identification and Expression Analysis of CSase Gene Family in Wheat

作　　者：冯永佳 王燕 李杰 李培华 刁邓超 李云丽 孙玉晨 孟祥宇 李春莲[1] 吴建辉 曾庆东[1] 韩德俊[1] 冯帆[1] 郑炜君[1] FENG Yongjia;WANG Yan;LI Jie;LI Peihua;DIAO Dengchao;LI Yunli;SUN Yuchen;MENG Xiangyu;LI Chunlian;WU Jianhui;ZENG Qingdong;HAN Dejun;FENG Fan;ZHENG Weijun(College of Agronomy/State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院/作物抗逆与高效生产国家重点实验室,陕西杨凌712100

出　　处：《麦类作物学报》2024年第12期1505-1514,共10页Journal of Triticeae Crops

基　　金：陕西省重点研发计划项目(2023-YBNY-026);科技创新2030重大项目(2023ZD04026);杨凌职业技术学院院士工作室项目(YS2023-005)。

摘　　要：半胱氨酸合成酶(CSase)基因家族在植物的生长发育和非生物胁迫抗性方面发挥重要作用。为了探究TaCSase在高温下的的表达特性,通过生物信息学方法对小麦CSase家族成员进行全基因组鉴定和分析,并通过转录组数据以及转化酵母初步研究其耐热功能。结果表明,小麦基因组鉴定出29个TaCSase基因,分布在17条染色体上,可划分为6个亚组,相同亚组内的成员具有相似的理化性质、基因结构和保守基序。共线性分析表明,TaCSase在小麦中以片段复制作为进化驱动力,在不同物种间可能存在不同的进化方式。顺式作用元件和GO富集分析发现,TaCSase含有大量非生物胁迫响应元件,在GO富集分析中TaCSase在半胱氨酸合成功能中被富集。qRT-PCR分析结果表明,TaCSase主要在根中表达,受高温胁迫诱导。酵母转化试验表明,转化SCS和CysB亚组成员的转基因酵母在高温下具有更好的生长势。这说明小麦TaCSase基因具有耐热功能,可用于小麦耐热育种。Cysteine synthase(CSase)gene gamily plays a crucial role in plant growth,development,and abiotic stress resistance.To explore the expression characteristics of TaCSase under high temperatures,we conducted a genome-wide identification and analysis of wheat CSase family members using bioinformatics methods.Additionally,we performed preliminary studies on their heat tolerance through transcriptome data analysis and yeast transformation experiments.A total of 29 TaCSase genes were identified in the wheat genome,distributed across 17 chromosomes and classified into six subgroups.Genes within the same subgroup exhibited similar physicochemical properties,gene structures,and conserved motifs.Collinearity analysis indicated that TaCSase evolved through segmental duplication in wheat,potentially adopting different evolutionary mechanisms across species.Cis-regulatory element analysis and GO enrichment showed that TaCSase members contain numerous abiotic stress-responsive elements and are enriched in cysteine biosynthesis functions.qRT-PCR results indicated that TaCSase is predominantly expressed in roots and is induced by heat stress.Yeast transformation experiments demonstrated that transgenic yeast harboring members of the SCS and CysB subgroups exhibited enhanced growth under high temperatures.It indicated that the wheat TaCSase genes had heat tolerance function and could be used in wheat breeding of heat telerance.

关 键 词：小麦 半胱氨酸合成酶 基因家族 高温胁迫 

分 类 号：S512.1[农业科学—作物学] S330