褪黑素引发对小麦种子萌发期和苗期抗旱性的影响  

Effects of Seeds Priming with Melatonin Solution on Drought Tolerance of Wheat during Germination and Seedling

作　　者：李波 昝亚玲[1] 杨青珍[1] 王霞[1] 孙晶 LI Bo;ZAN Yaling;YANG Qingzhen;WANG Xia;SUN Jing(Department of Life Science,Yuncheng University,Yuncheng,Shanxi 044000,China;Yuncheng Science and Technology Bureau,Yuncheng,Shanxi 044000,China)

机构地区：[1]运城学院生命科学系,山西运城044000 [2]运城市科学技术局,山西运城044000

出　　处：《麦类作物学报》2024年第12期1630-1638,共9页Journal of Triticeae Crops

基　　金：山西省基础研究计划项目(202103021223372);运城市基础研究计划项目(YCKJ-2022071);山西省高等学校科技创新项目(2021L478);山西省高等学校科技创新计划创新实验室平台项目(2022P012)。

摘　　要：为了解褪黑素对小麦种子萌发期和苗期抗旱性的调节作用,以小麦品种运旱20410为试验材料,测定和分析了不同浓度(10、50、100、300、500和800μmol·L^(-1))褪黑素引发处理后在聚乙二醇(PEG)胁迫条件下小麦种子萌发指标和幼苗生长参数的变化。结果表明,在PEG胁迫条件下,10~800μmol·L^(-1)褪黑素引发增强了小麦种子发芽能力,缓解了PEG胁迫对幼苗生长发育的抑制作用,促进了种子萌发和植株生长。随着褪黑素浓度的升高,种子发芽势、发芽率、发芽指数和活力指数均呈先升后降趋势,以100μmol·L^(-1)褪黑素处理的种子发芽力和活力最强,引发效果最明显;各褪黑素处理的幼苗根长、株高和鲜重均显著大于对照(无褪黑素),但不同浓度褪黑素处理间无明显差异。10~300μmol·L^(-1)褪黑素引发提高了干旱胁迫下苗期植株抗旱性,加速脯氨酸积累的同时降低了丙二醛(MDA)含量,其中100μmol·L^(-1)褪黑素处理下叶片中脯氨酸和MDA积累量分别最高或最低。由此可见,褪黑素引发可增强小麦种子萌发期和苗期的抗旱性,以100μmol·L^(-1)褪黑素效果最佳。In order to understand the regulation of melatonin(MT)to the drought resistance of wheat at seed germination stage and seedling stage,‘Yunhan 20410’,a wheat variety,was used as experimental material to analyze the change of some seed germination and seedling growth indexes after the treatment of different concentrations(10,50,100,300,500,and 800μmol·L^(-1))of MT under polyethylene glycol(PEG)stress.The results indicated that MT treatment enhanced wheat seed germination ability and promoted seed germination under PEG stress.With the increased of MT solution concentration,the germination potential,germination rate,germination index,and vigor index within the range were increased and then decreased.Among them,seed priming with 100μmol·L^(-1)MT showed the best effect.MT-priming drastically eliminated the inhibition of PEG stress on plant growth.Meanwhile,wheat treated with different concentration MT exhibited longer root length,higher plant height,and higher fresh weight under PEG stress,but there was no significant difference among the treatments.Moreover,MT pretreatment with 10-300μmol·L^(-1)enhanced drought resistance in wheat plant.Measurement of physiological parameters showed an increase in proline contents,with a decrease in malondialdehyde(MDA)content in leaves from wheat seedlings with MT-priming when exposed to drought stress.The seeds primed with 100μmol·L^(-1)MT performed the highest accumulation of proline and lowest MDA content in leaves.In summary,seed priming with MT can enhance the drought tolerance of wheat at seed germination and seedling growth stages,and the effect of 100μmol·L^(-1)MT is best.

关 键 词：褪黑素 种子引发 小麦 种子萌发 幼苗生长 抗旱性 

分 类 号：S512.1[农业科学—作物学] S311