男性精子畸形率及DNA完整性与复发性流产的相关性  

Correlation between male sperm deformity rate and DNA integrity and recurrent abortion

作　　者：张杰[1] 吴斌[1] 祝静[1] 王静[1] 刘启航 陈小芬[1] ZHANG Jie;WU Bin;ZHU Jing;WANG Jing;LIU Qihang;CHEN Xiaofen(Department of Reproductive Medicine,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China)

机构地区：[1]川北医学院附属医院生殖医学科,四川南充637000

出　　处：《新乡医学院学报》2024年第12期1159-1164,共6页Journal of Xinxiang Medical University

基　　金：四川省自然科学基金资助项目(编号:2022NSFSC1357)。

摘　　要：目的探讨男性精子畸形率及DNA完整性与复发性流产的相关性。方法选择2019年8月至2021年8月川北医学院附属医院收治的45例复发性流产患者及其丈夫为复发性流产组,另选择同期于川北医学院附属医院建档与产检的45例产妇及其丈夫作为正常妊娠组。统计2组女性受试者的年龄、体质量指数(BMI)、流产次数、子宫状况(宫腔粘连、内膜息肉、不全纵隔、正常)、合并高血压、合并糖尿病等资料。90例男性禁欲2~7 d后采用体外排精法收集排出的全部精液。使用全自动精子质量分析仪检测2组男性受试者的精液量、pH值、精子总活力、前向运动精子(PR)百分率及精子浓度,采用精子形态学快速染色法观察2组男性受试者的精子形态并计算精子畸形率,使用基于流式细胞术的精子染色质结构分析方法计算2组男性受试者的精子DNA碎片化指数;比较2组男性受试者的精液量、pH值、PR百分率、精子总活力、精子浓度、精子畸形率及精子DNA碎片化指数。复发性流产组男性受试者接受密度梯度离心法精子优选,比较精子优选前后的精液量、pH值、PR百分率、精子总活力、精子浓度、精子畸形率及精子DNA碎片化指数指标变化;复发性流产组采取宫腔内人工授精治疗,记录临床妊娠情况;采用双相关变量Spearman分析男性精子总活力、PR百分率、精子浓度、精子畸形率及精子DNA完整性与复发性流产发生的关系。结果复发性流产组与正常妊娠组女性受试者的年龄、BMI、子宫状况、合并高血压与糖尿病占比比较差异无统计学意义(P>0.05)。复发性流产组男性受试者的PR百分率、精子总活力及精子浓度显著低于正常妊娠组(P<0.05);复发性流产组与正常妊娠组男性受试者的精液量、pH值比较差异无统计学意义(P>0.05)。复发性流产组男性受试者的精子头部畸形率及总畸形率高于正常妊娠组(P<0.05);�Objective To investigate the correlation between male sperm deformity rate and DNA integrity and recurrent spontaneous abortion.Methods Forty-five patients with recurrent spontaneous abortion and their husbands who were admitted to the Affiliated Hospital of North Sichuan Medical College from August 2019 to August 2021 were selected as the recurrent abortion group,and another 45 parturients and their husbands who were documented and examined during the same period in the Affiliated Hospital of North Sichuan Medical College were selected as the normal pregnancy group.The age,body mass index(BMI),number of abortions,uterine status(such as intrauterine adhesions,endometrial polyps,incomplete septum,normal),combined hypertension,and combined diabetes were recorded for female subjects in the two groups.After abstinence for 2 to 7 days,all the excreted semen of the 90 male subjects were collected by extracorporeal sperm collection.The semen volume,pH value,total sperm motility,forward motile sperm(PR)percentage,and sperm concentration of male subjects in the two groups were detected by using an automatic sperm quality analyzer.The sperm morphology was observed by using the Diff-Quik method,and the sperm deformity rate was calculated.The sperm DNA fragmentation index of male subjects in the two groups was calculated by using a flow cytometry-based sperm chromatin structure analysis method.The semen volume,pH value,PR percentage,total sperm motility,sperm concentration,sperm deformity rate,and sperm DNA fragmentation index of male subjects between the two groups were compared.The male subjects in the recurrent abortion group were subjected to density gradient centrifugation for sperm selection,and the changes in semen volume,pH value,PR percentage,total sperm motility,sperm concentration,sperm deformity rate,and sperm DNA fragmentation index before and after sperm selection were compared.Intrauterine insemination was performed and the clinical pregnancy was recorded in the recurrent abortion group.The double correlated v

关 键 词：复发性流产 精子畸形率 DNA完整性 人工授精 

分 类 号：R698.2[医药卫生—泌尿科学] R714.21[医药卫生—外科学]