大葱离体雌核培养及植株再生的研究  

Study on Vitro Female Culture and Plant Regeneration in Allium fistulosum L.

作　　者：李娜[1] 贾俊香[1] 杨国栋[1] 蒋欣陶 姜滨滨[1] 崔连伟[1] LI Na(Vegetables Research Institute,Liaoning Academy of Agriculture,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院蔬菜研究所,辽宁沈阳110161

出　　处：《园艺与种苗》2024年第11期3-4,共2页Horticulture & Seed

基　　金：上海市农业科技创新项目(沪农科B2023001);国家特色蔬菜产业技术体系-大葱品种改良岗位项目(CARS-24-A-08)。

摘　　要：[目的]研究大葱胚状体及其再生植株的诱导方法。[方法]以大葱花蕾为试材,利用正交试验法对大葱离体雌核培养进行了相关研究。[结果]6-BA、NAA和2,4-D对诱导雌核出胚有极显著影响。[结论]添加6-BA 2.5 mg/L、NAA 0.04 mg/L、2,4-D 1.0 mg/L为最优诱导培养基,雌核培养再生植株成活率可达78.2%。[Objective]To study the induction of embryoid and regenerated plants from buds of Allium fistulosum L..[Method]In vitro gynogenetic culture of A.fistulosum L.was studied by orthogonal test.[Result]The results showed that 6-BA,NAA and 2,4-D had significant effects on gynogenesis.[Conclusion]The optimum induction medium was 6-BA 2.5 mg/L,NAA 0.04 mg/L and 2,4-D 1.0 mg/L.The survival rate of regenerated plants by gynogenetic culture can reach 78.2%.

关 键 词：大葱 离体 正交试验 雌核培养 再生 

分 类 号：S633.1[农业科学—蔬菜学]