基于分形传播方法的光片显微镜组织成像仿真  

Simulation of Tissue Imaging in Light-Sheet Microscopy Based on Fractal Propagation Method

作　　者：王梓豪 杨孝全 江涛 Wang Zihao;Yang Xiaoquan;Jiang Tao(School of Biomedical Engineering,Hainan University,Haikou 570228,Hainan,China;HUST-Suzhou Institute for Brainsmatics,Suzhou 215000,Jiangsu,China)

机构地区：[1]海南大学生物医学工程学院,海南海口570228 [2]华中科技大学苏州脑空间信息研究院,江苏苏州215000

出　　处：《激光与光电子学进展》2024年第20期177-186,共10页Laser & Optoelectronics Progress

基　　金：国家重点研发计划(2022YEF0203200)。

摘　　要：光片显微镜因具有高通量三维层析和适合长时程活体成像等优点,在生命科学领域得到了广泛应用。然而,由于光散射的影响,不同光片显微镜的成像性能在散射介质中的表现尚未明确。为填补这一研究空白,本文以物镜后焦平面电场为出发点,采用分形传播方法推导光在非均质散射介质中的传播,再利用伴随方法建立适用于光片显微镜的双物镜形式仿真架构。通过结合不同光片显微镜中的扫描和探测方式,推导出图像形成过程,实现了4种光片显微镜的三维成像模拟。分析不同光片显微镜在不同光学长度的仿真模型中成像的分辨率和对比度,结果表明:光片显微镜的抗散射能力与显微镜共焦性、光片厚度相关,与狭缝大小无关,且探测端相比照明端更易受散射影响。Light-sheet microscopy has become a pivotal tool in life sciences,offering benefits such as high-throughput 3D tomography and suitability for long-term in vivo imaging.Despite these advantages,the impact of light scattering on imaging performance across different optical microscopes in scattering media remains poorly understood.This study addresses this knowledge gap by considering the electric field at the back focal plane of objective.It employs the fractal propagation method to derive the light propagation in heterogeneous scattering media and utilizes the adjoint method to develop a dual objective simulation architecture for optical microscopy.By integrating various scanning and detection strategies from different optical microscopes,the image formation process was elaborated,facilitating three-dimensional imaging simulations across four distinct types of optical microscopes.The study reveals that the capability of light-sheet microscopy to retain image quality is correlated with the confocal properties of the microscope and the thickness of the light sheet,rather than the size of the slit and the detection end exhibits greater susceptibility to scattering effects than the illumination end.

关 键 词：光片显微镜 分形传播方法 散射介质模型 成像仿真 

分 类 号：O436[机械工程—光学工程]