云贵川地区条形柄锈菌小麦专化型群体遗传分析  

Population genetics analysis of Puccinia striiformis f.sp.tritici in Yunnan-Guizhou-Sichuan area

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作  者:张志博 刘迪 刘瀚铮 张思玥 赵璟琛 张吉光[1] 李宇翔 胡小平[1] 商文静[1] ZHANG Zhibo;LIU Di;LIU Hanzheng;ZHANG Siyue;ZHAO Jingchen;ZHANG Jiguang;LI Yuxiang;HU Xiaoping;SHANG Wenjing(Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区:[1]西北农林科技大学植物保护学院,植保资源与病虫害治理教育部重点实验室,农业农村部西北黄土高原作物有害生物综合治理重点实验室,陕西杨凌712100

出  处:《菌物学报》2024年第11期89-101,共13页Mycosystema

基  金:国家重点研发计划(2021YFD1401000);科技部国际合作项目(G2023172013L);陕西省自然科学基础研究计划(2024JC-YBQN-0197)。

摘  要:云贵川地区是我国小麦条锈病的常发区,明确我国云贵川地区条形柄锈菌小麦专化型Puccinia striiformis f.sp.tritici遗传结构和菌源交流关系,对该地区及流行区小麦条锈病的防治具有重要意义。本研究以2022年采自云南、贵州和四川的203株条形柄锈菌小麦专化型菌株为试验材料,利用25对KASP-SNP引物完成基因型分析。根据KASP-SNP分子数据进行遗传聚类分析结果表明,贵州西部群体与云南中部群体分布更相似,遗传距离更近。群体遗传结构分析表明除四川盆地群体外,其他群体的遗传结构相似。主成分判别分析进一步说明四川盆地群体相对独立,与其他群体菌源交流较少,除四川盆地群体外的各群体之间存在频繁的菌源交流。群体遗传分化分析显示四川盆地群体与云南东北部-凉山州群体FST最大,为0.043,两者遗传差异最大。贵州西部群体与云南中部群体遗传分化程度最低,为0.003。通过基因流分析显示,各群体之间基因流(Nm)均大于4,表明各群体之间存在频繁的基因交流。遗传多样性分析表明四川盆地群体与贵州西部群体遗传多样性水平较高,香农信息指数分别为0.527和0.487。本研究证明我国云贵川地区内部既存在频繁的菌源交流,又存在相对独立的地区,云南和贵州的条形柄锈菌小麦专化型可能作为1个大群体在我国小麦条锈病菌的传播中发挥作用。Yunnan-Guizhou-Sichuan area is a common occurrence area of wheat stripe rust in China.It is greatly significant to clarify the genetic structure and inoculum source exchange relationship of Puccinia striiformis f.sp.tritici(Pst)in this endemic area.In this study,203 samples of the pathogen collected from this area in 2022 were used as experimental materials,and 25 pairs of KASP-SNP primers were used to complete the genotype analysis.The results of genetic cluster analysis using KASP-SNP molecular data showed that the distribution of western Guizhou population of the pathogen was more similar to that of central Yunnan population,and the genetic distance was closer.Structure analysis showed that except for Sichuan basin population,the genetic structure of other populations was similar.DAPC analysis further showed that the Sichuan basin population was relatively independent and had less interchange with other population,while all populations except Sichuan basin population frequently exchanged genetically each other.The analysis of population genetic differentiation showed that the FST of Sichuan basin population and northeastern Yunnan-Liangshanzhou population was the largest(FST=0.043),and the genetic difference between them was the largest.The genetic differentiation degree of western Guizhou population and that of central Yunnan population was the lowest(FST=0.003).The gene flow among populations was>4,indicating that there were frequent gene exchanges among populations.Genetic diversity level of Sichuan basin population and that of western Guizhou population was relatively high,with Shannon information index of 0.527 and 0.487 respectively.It was proved that there were frequent inoculum source exchanges of wheat stripe rust pathogen in Yunnan-Guizhou-Sichuan area,but there was exceptional independent area relatively lacking gene exchange.It was speculated that the pathogen in Yunnan and Guizhou may act as a large population in the transmission of wheat stripe rust in China.

关 键 词:条形柄锈菌小麦专化型 KASP-SNP 群体遗传 云贵川地区 

分 类 号:S435.121.42[农业科学—农业昆虫与害虫防治]

 

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