MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis  

作　　者：Tianqi Qiu Yuanyuan Su Nannan Guo Xinyuan Zhang Pengfei Jia Tonglin Mao Xianling Wang 

机构地区：[1]College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China [2]College of Biological Science,China Agricultural University,Beijing 100091,China

出　　处：《Journal of Integrative Plant Biology》2024年第11期2379-2394,共16页植物学报（英文版）

基　　金：supported by National Natural Science Foundation of China(31970661 and 32370736);Liaoning Education Foundation(JYTQN2024007);Young and Middle-aged Science and Technology Innovation Talent Support Program of Shenyang(RC220273)。

摘　　要：It has been proposed that cortical fine actin filaments are needed for the morphogenesis of pavement cells(PCs).However,the precise role and regulation mechanisms of actin filaments in PC morphogenesis are not well understood.Here,we found that Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTOR9(ADF9) is required for the morphogenesis of PC,which is negatively regulated by the R2R3 MYELOBLASTOSIS(MYB) transcription factor MYB52.In adf9 mutants,the lobe number of cotyledon PCs was significantly reduced,while the average lobe length did not differ significantly compared to that of wild type(Col-0),except for the variations in cell area and circularity,whereas the PC shapes in ADF9 overexpression seedlings showed different results.ADF9 decorated actin filaments,and colocalized with plasma membrane.The extent of filament bundling and actin filament bundling activity in adf9 mutant decreased.In addition,MYB52 directly targeted the promoter of ADF9 and negatively regulated its expression.The myb52-2 mutant showed increased lobe number and cell area,reduced cell circularity of PCs,and the PC phenotypes were suppressed when ADF9 was knocked out.Taken together,our data demonstrate that actin filaments play an important role in the morphogenesis of PC and reveal a transcriptional mechanism underlying MYB52 regulation of ADF9-mediated actin filament bundling in PC morphogenesis.

关 键 词：actin filament bundling ADF9 MYB52 pavement cell 

分 类 号：Q943.2[生物学—植物学]