Natural variations in MdNAC18 exert major genetic effect on apple fruit harvest date by regulating ethylene biosynthesis genes  

作　　者：Guo Wen Bei Wu Yi Wang Ting Wu Zhenhai Han Xinzhong Zhang 

机构地区：[1]College of Horticulture,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Integrative Plant Biology》2024年第11期2450-2469,共20页植物学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(U22A20490);the Modern Agricultural Industry Technology System(CARS-27);the Key Research and Development Program of Hebei(21326353D;21326308D)。

摘　　要：Dissecting the genetic control of apple fruit harvest date(AFHD) into multiple Mendelian factors poses a significant challenge in modern genetics.Here,a quantitative trait locus(QTL)for AFHD was fine-mapped to the NAC transcription factor(TF) MdNAC18 within the interval defined by the overlap of QTLs Z03.5/Z03.6 and F03.2/F03.3.One direct target of MdNAC18 is the ethylene biosynthesis gene MdACO1.The single nucleotide polymorphisms(SNPs) SNP517 and SNP958 in the MdNAC18 coding sequence modulated activation of MdACO1by MdNAC18.SNP1229 in the MdACO1 promoter destroyed the MdNAC18 binding site and thus abolished MdNAC18 binding.SNP517 and SNP958also affected MdNAC18 activation of the TF gene MdARF5;MdARF5 activates the ethylene biosynthesis gene MdACS1.SNP517 and SNP958 in MdNAC18,SNP1229 and SNP769(linked to In Del62) in MdACO1,and In Del162 in MdACS1constituted a genetic variation network.The genetic effect of this network on AFHD was estimated as60.3 d,accounting for 52.6% of the phenotype variation of the training population.The joint effects of these polymorphisms increased the accuracy of a genomics-assisted prediction(GAP) model for AFHD(r = 0.7125).Together,our results suggest that genetic variation in MdNAC18 affects AFHD by modulating ethylene biosynthesis and provide an optimized GAP model for apple breeding.

关 键 词：ETHYLENE fruit harvest date genetic variation Malus domestica Borkh MdNAC18 

分 类 号：S661.1[农业科学—果树学]