4取代三联吡啶钴和镍的抗菌活性研究  

Study on antibacterial activity of 4-substituted tripyridyl cobalt and nickel

作　　者：罗家其 马成 黄清萍 谢芝港 江明生[1] 刘宏鸣 陈海兰[1,3,4] LUO Jia-qi;MA Cheng;HUANG Qing-ping;XIE Zhi-gang;JIANG Ming-sheng;LIU Hong-ming;CHEN Hai-lan

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西大学化学与化工学院,广西南宁530004 [3]广西壮族自治区兽用生物制品工程研究中心,广西南宁530004 [4]广西畜禽繁育与疾病防控重点实验室,广西南宁530004

出　　处：《饲料研究》2024年第20期86-90,共5页Feed Research

基　　金：大学生创新创业训练项目(项目编号:202310593451);巴马人才科技专项(项目编号:202101267)。

摘　　要：试验旨在研究4取代三联吡啶钴和镍配合物的抗菌活性及机制。试验采用二倍稀释法测定10种不同的4取代三联吡啶钴和镍金属配合物对6种常见致病菌(肠炎沙门氏菌、金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌K99、无乳链球菌和副溶血弧菌)的最小抑菌浓度(MIC),分析其抗菌活性。结果显示,配合物对金黄色葡萄球菌的抑菌效果较好,MIC值普遍较低,其中LB3 NiCl_(2)对金黄色葡萄球菌的MIC低至5 mg/L;金黄色葡萄球菌的生长曲线和致死曲线显示,LB3 NiCl_(2)在不同程度上抑制细菌的生长,且具有浓度依赖性;荧光显微镜观察结果显示,金属配合物处理后细菌细胞膜通透性增强。研究表明,4取代三联吡啶钴和镍配合物对金黄色葡萄球菌具有良好的抗菌活性。The experiment aimed to study the antibacterial activity and mechanism of 4-substituted terpyridine cobalt and nickel complexes.The experiment used the two-fold dilution method to determine the minimum inhibitory concentration(MIC)of 10 different 4-substituted terpyridine cobalt and nickel metal complexes against six common pathogenic bacteria(Salmonella enterica,Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli K99,Streptococcus agalactiae,and Vibrio parahaemolyticus),and analyzed their antibacterial activity.The results showed that the complexes had good inhibitory effects on Staphylococcus aureus,with generally low MIC values,among which the MIC of LB3 NiCl_(2) against Staphylococcus aureus was as low as 5 mg/L.The growth curve and death curve of Staphylococcus aureus showed that LB3 NiCl_(2) inhibited bacterial growth to varying degrees and had concentration dependence.The results of fluorescence microscopy observation showed that the permeability of bacterial cell membranes increased after treatment with metal complexes.The study indicates that 4-substituted terpyridine cobalt and nickel complexes have good antibacterial activity against Staphylococcus aureus.

关 键 词：三联吡啶 镍配合物 抗菌活性 金黄色葡萄球菌 

分 类 号：S816.7[农业科学—饲料科学]