甘草渣中甘草总黄酮和甘草查尔酮A的含量测定及提取纯化工艺优化  

Content Determination of Licoflavone and Licochalcone A in Licorice Residues and Optimization in Extraction and Purification Process

作　　者：张周 杨君义 王晓霞 王云 刘柔鑫 陈文 李红 ZHANG Zhou;YANG Junyi;WANG Xiaoxia;WANG Yun;LIU Rouxin;CHEN Wen;LI Hong(Key Laboratory of Xinjiang Phytomedicine Resources and Utilization,Ministry of Education,School of Pharmacy,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学药学院新疆植物药资源利用教育部重点实验室,新疆石河子832003

出　　处：《化学与生物工程》2024年第12期55-61,共7页Chemistry & Bioengineering

基　　金：石河子大学科研计划项目(CXFZ202114)。

摘　　要：以野生胀果甘草为研究对象,以甘草苷为对照品,建立了紫外可见分光光度法测定甘草总黄酮含量,同时建立了UPLC法测定甘草查尔酮A含量;以甘草总黄酮和甘草查尔酮A的含量为评价指标,采用单因素实验和正交实验考察了提取时间、料液比、乙醇体积分数、提取温度对甘草渣中甘草总黄酮和甘草查尔酮A含量的影响;采用超滤膜技术纯化提取液中的甘草总黄酮和甘草查尔酮A,考察了不同截留分子量的超滤膜对甘草总黄酮和甘草查尔酮A含量的影响。结果表明,紫外可见分光光度法测定甘草总黄酮含量的线性范围为3.806~13.312μg·mL^(-1),UPLC法测定甘草查尔酮A含量的线性范围为9.175~73.400μg·mL^(-1);最优提取工艺为:提取时间115 min、料液比1∶20(g∶mL)、乙醇体积分数75%、提取温度90℃;采用截留分子量为8 kDa的超滤膜对甘草渣提取液进行纯化,甘草总黄酮的平均含量提高了6倍左右,甘草查尔酮A的平均含量提高了9倍左右。该研究为野生胀果甘草渣中甘草总黄酮和甘草查尔酮A的提取纯化提供了一定实验依据。Using wild Glycyrrhiza inflata Bat.as the research object and glycyrrhizin as a reference substance,we established a UV-Vis spectrophotometry method to determine the contents of licoflavone,and a UPLC method to determine the contents of licochalcone A.Moreover,using the contents of licoflavone and licochalcone A as evaluation indexes,we investigated the effects of extraction time,solid-liquid ratio,ethanol volume fraction,and extraction temperature on the contents of licoflavone and licochalcone A in licorice residues by single-factor experiments and orthogonal experiments.Furthermore,we investigated the effect of ultrafiltration membranes with different molecular weight cutoffs on the contents of licoflavone and licochalcone A in licorice residues.The results show that the linear range for the determination of licoflavone in licorice residues by UV-Vis spectrophotometry is 3.806-13.312μg·mL^(-1),and the linear range for the determination of licochalcone A in licorice residues by UPLC is 9.175-73.400μg·mL^(-1).The optimal extraction process is obtained as follows:the extraction time of 115 min,the solid-liquid ratio of 1∶20(g∶mL),the ethanol volume fraction of 75%,and the extraction temperature of 90℃.The purification of the extraction solution of licorice residues is carried out by using ultrafiltration membrane with a molecular weight cutoff of 8 kDa.The average content of licoflavone in licorice residues increases by about 6 times,and the average content of licochalcone A in licorice residues increases by about 9 times.This study provides experimental evidence for the extraction and purification of licoflavone and licochalcone A from wild Glycyrrhiza inflata Bat.residues.

关 键 词：甘草渣 甘草总黄酮 甘草查尔酮A 超滤膜 

分 类 号：R284[医药卫生—中药学]