云新系核桃脂肪酸合成和种仁性状与酶活性相关性研究  

Study on Fatty Acid Synthesis and Related Enzyme Activity of Yunxin Walnut

作　　者：朱秋蓉 赵壮 和润喜[3] 石卓功[3] ZHU Qiu-rong;ZHAO Zhuang;HE Run-xi(The Tropical Forestry Research Institute of Yunnan Academy of Forestry and Grassland,Puwen,Yunnan 666102;Qiubei County No.4 Middle School,Qiubei,Yunnan 663200;Southwest Forestry University,Kunming,Yunnan 650224)

机构地区：[1]云南省林业和草原科学院热带林业研究所,云南普文666102 [2]丘北县第四中学,云南丘北663200 [3]西南林业大学,云南昆明650224

出　　处：《安徽农业科学》2024年第23期111-114,共4页Journal of Anhui Agricultural Sciences

基　　金：云南省重大科技专项“核桃优异种质评价”(2018ZG002-1)。

摘　　要：为了解云新系核桃脂肪酸合成、缩仁率等种仁性状与相关酶活性的关系,以“云新高原”和“云新301”2个云新系品种为试验材料,研究果实在不同成熟期脂肪酸合成相关酶的活性情况。结果表明:种仁中植物乙酰辅酶A羧化酶(ACCase)、植物丙酮酸激酶(PK)、植物6-磷酸葡萄糖脱氢酶(G6PDH)3种脂肪酸合成相关酶的活性从硬核期至果实近成熟期均表现为先升高后降低。在脂肪酸组分中,成熟核仁中含量最高的是亚油酸,达56.30%,其次是油酸24.49%,α-亚麻酸8.67%,棕榈酸7.81%,硬脂酸含量最低2.73%。总体来说,所测云新系核桃中的油脂组分不饱和脂肪酸含量远高于饱和脂肪酸含量,5种脂肪酸与3个脂肪酸合成相关酶活性之间虽无显著相关关系,但是所测脂肪酸组分之间具有显著的相关关系。ACCase、PK、G6PDH 3种酶活性在核桃脂肪合成过程中均有重要作用,ACCase、PK 2种酶活性是影响核桃种仁脂肪含量的关键,也是影响核桃缩仁的关键因素。In order to understand the relationship between seed traits such as fatty acid synthesis and kernel shrinkage rate and related enzyme activity in Yunxin walnut,two Yunxin cultivars,‘Yunxin plateau’and‘Yunxin 301’,were used as experimental materials to study the activity of fatty acid synthesis related enzymes in fruits at different maturity stages.Results showed that:the activity of 3 kinds of fatty acid synthesis related enzymes in the seed acelase A carboxyrase(ACCASE),plant acetone kinase(PK),plant 6-phosphoric acid synthetic enzymes The near-mature period is manifested as a rise first and then decrease.Among the fatty acid components,the highest content of linoleic acid was 56.30%,followed by oleic acid was 24.49%,α-linolenic acid was 8.67%,palmitic acid was 7.81%and stearic acid was 2.73%.Generally speaking,the content of unsaturated fatty acids in the measured clouds in the new line of clouds is much higher than the content of saturated fatty acids.There is no significant correlation between 5 fatty acids and 3 fatty acid synthesis activity,but the measured is measured,but the measured measured There is a significant correlation between fatty acids.The three enzymes of ACCASE,PK,and G6PDH play an important role in the process of walnut fat synthesis.The two enzyme activity of ACCASE and PK is the key to affecting the content of walnut kernels and the key factor affecting walnut shrinkage.

关 键 词：云新系核桃 脂肪酸 脂肪酸合成酶 

分 类 号：S664.1[农业科学—果树学]