紫薇种子无菌萌发与快繁技术研究  

Study on Aseptic Germination and Rapid Propagation of Lagerstroemia indica Seeds

作　　者：闫娜[1] 王春彦[1] 华雨欣 何岭 YAN Na;WANG Chun-yan;HUA Yu-xin(Jinling Institute of Technology,Nanjing,Jiangsu 210038)

机构地区：[1]金陵科技学院,江苏南京210038

出　　处：《安徽农业科学》2024年第23期118-121,128,共5页Journal of Anhui Agricultural Sciences

基　　金：2021年南京市绿化园林局科技项目(YLKJ202111JH)。

摘　　要：以花瓣深粉红色的紫薇品种“彩霞满天”(Lagerstroemia indica‘Caixia Mantian’)的自然结实种子为试验材料,建立种子无菌萌发体系,以试管苗探索基本培养基类型、植物生长调节剂种类及浓度对不定芽增殖和根系诱导的影响,筛选出无菌苗“一步法”增殖、生根培养基。结果表明:紫薇种子无菌萌发最佳消毒方案为先用75%乙醇消毒2 min后,用2.5%NaClO消毒5 min;相同灭菌方案下接种于WPM培养基的紫薇较接种于MS的紫薇褐化率总体偏少,且较接种于1/2MS和MS中无菌萌发苗的茎粗壮且叶色浓绿;筛选出紫薇“一步法”增殖、生根最佳培养基为WPM+6-BA 1.5 mg/L+IBA 0.05 mg/L,试管苗叶色浓绿,增殖系数达到3.09,平均芽长为5.21 cm,平均生根率达75.00%,单株平均生根数为2.78。In this study,the naturally fertile seeds of Lagerstroemia indica‘Caixia Mantian’with dark pink petals were used as test material to establish the seed aseptic germination system,the effects of basic medium type,plant growth regulator type and concentration on adventitious bud proliferation and root induction were explored with test-tube seedlings,and the one-step breeding and rooting medium of bacteria-free seedlings was screened.The results showed that the optimal disinfection scheme for the seed germ-free germination of Lagerstroemia was:disinfection with 75%alcohol for 2 min,then disinfection with 2.5%NaClO for 5 min;the browning rate of L.indica inoculated in WPM medium was lower than that inoculated in MS,and the stems were thicker and the leaves were darker green than those inoculated in 1/2MS and MS.The optimal medium for one-step proliferation and rooting was WPM+6-BA 1.5 mg/L+IBA 0.05 mg/L;the leaf color of the test tube seedlings was dark green,the proliferation coefficient reached 3.09,the average bud length was 5.21 cm,the average rooting rate was 75%,and the average rooting number per plant was 2.78.

关 键 词：紫薇 无菌萌发 离体培养 继代增殖 

分 类 号：S722[农业科学—林木遗传育种]