果生刺盘孢菌LFD-RPA可视化快速检测方法的建立  

The Visual Rapid Detection of Colletotrichum fructicola Based on LFD-RPA Assay

作　　者：潘锐 徐会永 臧昊昱 谷春艳 齐永杰[2,3,4] 杨雪 高正辉 PAN Rui;XU Hui-yong;ZANG Hao-yu(Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230001)

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,安徽合肥230001 [2]安徽省农业科学院园艺研究所,安徽合肥230001 [3]农业农村部园艺作物种质创制与利用重点实验室(部省共建),安徽合肥230001 [4]园艺作物种质资源创制与高效栽培安徽省重点实验室,安徽合肥230001

出　　处：《安徽农业科学》2024年第23期136-139,共4页Journal of Anhui Agricultural Sciences

基　　金：农业农村部园艺作物种质创制与利用重点实验室(部省共建)项目;国家梨产业技术体系专项(CARS-28);安徽省农业科学院平台项目(2023YL015);安徽省果树产业技术体系(皖农科函[2021]711号);园艺作物种质资源创制与高效栽培安徽省重点实验室项目(皖科基地秘[2023]430号)。

摘　　要：建立针对果生刺盘孢菌(Colletotrichum fructicola)的快速、准确、简便的检测方法。根据果生刺盘孢菌的基因序列设计了特异性检测引物和探针,建立了重组酶聚合酶扩增与侧流层析试纸条(LFD-RPA)相结合的可视化快速检测果生刺盘孢菌的方法。该检测方法与其他病原菌无交叉反应,特异性强,且检测灵敏度达1 pg/μL。该检测方法无需PCR仪等复杂设备,在39℃恒温反应12 min即可完成检测过程,为果生刺盘孢菌的检验检测和早期预警提供了技术支撑。In order to establish a rapid,accurate and simple detection method of Colletotrichum fructicola.A visual rapid detection assay of C.fructicola was established by designing specific primers and probe according to the gene sequence of C.fructicola based on the combination recombinase polymerase amplification with lateral flow dipsticks(LFD-RPA).The detection method had strong specificity and high sensitivity(1 pg/μL),besides,there was no cross-reaction with other pathogens.This method could be applied without any expensive and complex equipments such as PCR apparatus,meanwhile,the temperature of reaction condition was 39℃and detection time was less than 12 min.This study provided a quick and simple technology for the detection and early warning of Colletotrichum fructicola.

关 键 词：果生刺盘孢菌 LFD-RPA 可视化快速检测 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]