多指标响应面-满意度函数优化秦岭龙胆提取工艺研究  

Study on Optimization of Extraction Process from Gentiana apiata by Multi-index Response Surface-satisfaction Function

作　　者：许海燕[1] 侯敏娜[1] 陈衍斌 王珊[1] 刘艳红[1] 彭修娟[1] 王青[1] 刘峰[1,2] XU Hai-yan;HOU Min-na;CHEN Yan-bin(Medical College,Shaanxi Institute of International Trade,Xianyang,Shaanxi 712046;Shaanxi Buchang Pharmaceutical Co.,Ltd.,Xi’an,Shaanxi 710075)

机构地区：[1]陕西国际商贸学院医药学院,陕西咸阳712046 [2]陕西步长制药有限公司,陕西西安710075

出　　处：《安徽农业科学》2024年第23期162-166,215,共6页Journal of Anhui Agricultural Sciences

基　　金：陕西省教育厅青年创新团队建设科研计划项目(21JP011);陕西省科技厅项目(2021SF-365);国家大学生创新创业项目(S202113123008)。

摘　　要：[目的]优化秦岭龙胆中龙胆苦苷和獐牙菜苦苷的共提取条件。[方法]在单因素试验基础上选取最佳因素水平,采用响应面-满意度函数优选料液比、提取时间、超声频率对龙胆苦苷和獐牙菜苦苷提取率的影响。[结果]秦岭龙胆中2个成分的共提最优条件为提取时间26 min、料液比1∶21(g:mL)、超声频率58 kHz,在此条件下,龙胆苦苷、獐牙菜苦苷的提取率分别为0.312%、0.258%,分别与预测值(0.326%、0.270%)接近。[结论]响应面-满意度函数可用于秦岭龙胆中龙胆苦苷和獐牙菜苦苷共提取工艺的优化,该方法快速、简单、稳定可行。[Objective]To optimize the co-extraction conditions of gentiopicroside and swertiamarin from Gentiana apiata.[Method]On the basis of single factor experiments,the optimal factor level was selected,and the response surface-satisfaction function was used to optimize the effects of solid-liquid ratio,extraction time and ultrasound frequency on the extraction rates of gentiopicroside and swertiamarin.[Result]The optimal conditions for co-extraction of two components from Gentiana apiata in Qinling Mountains were extraction time of 26 minutes,solid-liquid ratio of 1∶21(g∶mL),and ultrasonic frequency of 58 kHz.Under these conditions,the extraction rates of gentiopicroside and swertiamarin were 0.312%and 0.258%,respectively,which were close to the predicted values of 0.326%and 0.270%,respectively.[Conclusion]The response surface satisfaction function can be used to optimize the co-extraction process of gentiopicroside and swertiamarin from Gentiana apiata in Qinling Mountains.This method is fast,simple,stable and feasible.

关 键 词：秦岭龙胆 龙胆苦苷 獐牙菜苦苷 响应面法 满意度函数 共提取条件 

分 类 号：R284.2[医药卫生—中药学]