辣椒PIN基因家族鉴定及其在果柄离区和根系发育中的表达分析  

Genome-wide identification and expression analysis of thePIN gene family in the pepper pedicel abscission zoneand during root development

作　　者：何磊 严希 赖卫 刘崇政 杨红[1] 何建文[1] HE Lei;YAN Xi;LAI Wei;LIU Chongzheng;YANG Hong;HE Jianwen(Institute of Pepper,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China)

机构地区：[1]贵州省农业科学院辣椒研究所,贵阳550025

出　　处：《西北植物学报》2024年第12期1878-1889,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：贵州省省级科技计划项目(黔科合基础-ZK[2022]一般220);贵州省省级科技计划项目(黔科合平台人才[2020]6018-2);贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023008);贵州省农业科学院项目(黔农科院青年基金[2022]21号)。

摘　　要：【目的】鉴定和分析辣椒PIN基因家族成员的特征及其在果柄离区和根系发育中的表达情况,为解析CaPINs功能及辣椒新品种培育提供候选基因。【方法】通过辣椒全基因组筛选鉴定PIN基因,并开展生物信息学分析,对这些基因在果柄离区和根系发育中进行表达分析。【结果】在辣椒基因组中鉴定出9个PIN基因家族成员,命名为CaPIN1-CaPIN9,分布于7条染色体上,CaPIN9未锚定。CaPINs蛋白的氨基酸数为358~654 aa,分子质量为39251.05~71170.91 D,等电点为6.40~9.38,且多数成员为稳定蛋白。CaPINs成员间具有明显的共线性,与番茄具有较近的遗传关系。CaPINs基因包含大量生长、环境及激素响应元件。转录组数据分析表明辣椒CaPINs的表达具有组织特异性。荧光定量PCR结果显示,CaPINs在不同果柄离区和根系发育中有不同程度表达。【结论】辣椒CaPINs特异性参与果柄离区形成与根系生长发育,可作为辣椒易脱柄品种培育和根系遗传改良的重要候选基因。[Objective]This study seeks to identify and analyze the PIN gene family members in pepper(Capsicum annuum L.)and to investigate their expression patterns in the abscission zone of the fruit pedicel as well as during root development.The ultimate goal is to provide candidate genes for elucidating the function of CaPINs and for breeding new pepper varieties.[Methods]PIN genes were identified through whole-genome screening of pepper, followed by comprehensive bioinformatics analysis. The expressionpatterns of these genes in the fruit pedicel abscission zone and during root development were systematicallyanalyzed. [Results] 9 members of the PIN gene family were identified in the pepper genome and designatedas CaPIN1 through CaPIN9. These genes are distributed across seven chromosomes, with CaPIN9remaining unanchored. The proteins encoded by these genes exhibit amino acid length ranging from 358 to654 residues, molecular weight from 39 251.05 D to 71 170.91 D, and isoelectric point from 6.40 to9.38. Most of these proteins are stable. Additionally, significant collinearity was observed among theCaPINs, indicating a close genetic relationship with the tomato genome. CaPINs encompass a multitudeof elements associated with growth, environmental responses, and hormonal signaling. Transcriptome dataanalysis revealed that the expression of CaPINs in pepper was tissue-specific. Quantitative PCR resultsdemonstrated that CaPINs exhibited differential expression levels across various fruit pedicel abscissionzones and throughout root development. [Conclusion] CaPINs play a crucial role in the development ofthe pedicel abscission zone and the growth and development of the root system. These genes hold significantpotential as candidates for breeding pepper varieties with improved ease of de-stemming and for enhancinggenetic traits related to root systems.

关 键 词：辣椒 果柄 PIN基因家族 生物信息学 表达分析 

分 类 号：Q943.2[生物学—植物学] S641.3[农业科学—蔬菜学]