蓖麻ZF-HD基因家族鉴定及表达模式分析  

Identification and expression analysis of the ZF-HD gene family in castor

作　　者：徐兴源 黄凤兰[2,3,4,5,6] 张春兰 朱梦洋[1] 崔志宇 刘鹏 XU Xingyuan;HUANG Fenglan;ZHANG Chunlan;ZHU Mengyang;CUI Zhiyu;LIU Peng(College of Agriculture,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;College of Life Sciences and Food Engineering,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission,Tongliao,Inner Mongolia 028000,China;Inner Mongolia Industrial Engineering Research Center of Universities for Castor,Tongliao,Inner Mongolia 028000,China;Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization,Tongliao,Inner Mongolia 028000,China;Castor Industry Technology Innovation Inner Mongolia Autonomous Region Engineering Research Center,Tongliao,Inner Mongolia 028000,China)

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028000 [2]内蒙古民族大学生命科学与食品学院,内蒙古通辽028000 [3]蓖麻育种国家民委重点实验室,内蒙古通辽028000 [4]内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽028000 [5]内蒙古自治区蓖麻育种与综合利用重点实验室,内蒙古通辽028000 [6]蓖麻产业技术创新内蒙古自治区工程研究中心,内蒙古通辽028000

出　　处：《西北植物学报》2024年第12期1915-1926,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31860389);蓖麻产业技术创新内蒙古自治区工程研究中心开放基金项目(MDK2023010);内蒙古自治区蓖麻产业协同创新中心开放基金项目(MDK2023078);“内蒙古自治区草原英才工程创新团队-蓖麻分子育种研究创新人才团队”滚动支持计划项目。

摘　　要：【目的】对蓖麻ZF-HD家族基因进行鉴定,并对其成员进行序列分析、系统进化分析、启动子及表达模式分析,为进一步研究蓖麻中ZF-HD基因的功能提供参考。【方法】以蓖麻全基因组数据为基础,从中鉴定出ZFHD基因,并进行生物信息学分析和非生物胁迫下的表达分析。【结果】从蓖麻中共鉴定到13个ZF-HD基因家族成员,该基因家族成员不均匀分布在Chr1-Chr10上,13个RcZF-HD蛋白均为亲水性不稳定蛋白,亚细胞定位于细胞核。RcZF-HD基因家族聚类为3个亚簇,且每簇基因数不同,基因结构具有簇内保守性和簇间多样性。Rc-ZF-HD基因家族成员具有大量组织特异性元件、应激响应元件和激素响应元件。ZF-HD基因广泛响应非生物胁迫,且表达具有组织特异性。【结论】在蓖麻中一共鉴定出13个ZF-HD基因。研究发现蓖麻ZF-HD基因家族成员在应对非生物胁迫时起重要作用,能够为蓖麻的抗逆性研究提供一定的理论依据。[Objective]The ZF-HD family genes of castor were identified and analyzed by sequence analysis,phylogenetic analysis,promoter and expression pattern analysis,to provide a reference for further study of the function of ZF-HD genes in castor.[Methods]Based on the whole genome data of castor,ZF-HD genes were identified,and bioinformatics and expression analysis under abiotic stress were carried out.[Results]A total of 13 ZF-HD genes were identified from castor,which were unevenly distributed on Chr1-Chr10.All 13 RcZF-HD proteins were hydrophilic unstable proteins,and were localized in the nucleus.The RcZF-HD genes were clustered into three subgroups,and the number of genes in each group was different.The gene structure was conserved within the group but diversified between groups.RcZF-HD genes had a large number of tissue-specific elements,stress response elements,and hormone response elements.The ZF-HD genes were widely responsive to abiotic stresses and their expression was tissue-specific.[Conclusion]A total of 13 ZF-HD genes were identified in castor.It is known that members of the castor ZF-HD gene family play an important role in responding to abiotic stress,this research provides theoretical basis for the study of castor’s stress resistance.

关 键 词：蓖麻 RcZF-HD基因家族 生物信息学 非生物胁迫 表达模式 

分 类 号：Q786[生物学—分子生物学] S565.6[农业科学—作物学]