西瓜雄性不育系花蕾不同发育时期生理特性的变化  

Physiological characteristics changes of flower buds of watermelon male sterile lines in different developmental stages

作　　者：高宁宁[1] 李晓慧[1] 康利允[1] 王琰 常高正[1] 李海伦 王慧颖 赵卫星[1] GAO Ningning;LI Xiaohui;KANG Liyun;WANG Yan;CHANG Gaozheng;LI Hailun;WANG Huiying;ZHAO Weixing(Horticulture Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002

出　　处：《中国瓜菜》2024年第12期60-65,共6页China Cucurbits And Vegetables

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-25);河南省农业科学院优秀青年科技基金项目(2022YQ12);河南省农业科学院自主创新资助项目(2024ZC031);河南省重大科技专项(221100110400)。

摘　　要：比较西瓜雄性不育株和可育株雄花蕾不同发育时期抗氧化酶活性、代谢物质及内源激素含量的变化,以探究西瓜雄性不育生理生化机制。以西瓜雄性不育系GMS4为试材,测定分析雄花蕾不同发育时期的生理生化指标。结果表明,在整个花蕾发育时期,不育株SOD活性和CAT活性整体下降,POD活性整体上升。不育株雄花蕾整个时期可溶性蛋白含量均低于可育株,脯氨酸含量不育株呈下降趋势,而可育株与其相反。不育株和可育株雄花花蕾中的IAA、ABA、ZR、GA3和JA等内源激素含量差异明显,且变化趋势不同。结果表明,抗氧化酶活性、代谢物质和内源激素含量的变化可能与西瓜花药败育有关。In order to explore the physiological and biochemical mechanism of male sterility in watermelon,the changes of antioxidant enzymes,metabolic substances and endogenous hormones in male flower buds of male sterile strains and fertile strains were compared at different developmental stages.Using the male sterile line GMS4 of watermelon as the test material,the physiological and biochemical indicators of male bud at different developmental stages were determined.The results showed that the SOD and CAT activities decreased overall and while the POD activity increased during the whole bud development period.The soluble protein content of male buds of sterile strains was lower than that of fertile strains,and the proline content of male buds of sterile strains showed a decreasing trend,while that of fertile strains was the opposite.The content of IAA,ABA,ZR,GA3 and JA endogenous hormones in male flower buds bewteen sterile and fertile strains were significantly different,and the change trend was different.The results showed that the changes of antioxidant enzyme activity,metabolic substances and endogenous hormone contents might be related to watermelon anther abortion.

关 键 词：西瓜 雄性不育 抗氧化酶 代谢物质 内源激素 

分 类 号：S651[农业科学—果树学]