当归芍药散基于海马组织谷氨酸和N-甲基-D-天冬氨酸受体对血管性痴呆影响的研究  

Study on the Effect of Danggui Shaoyao San on Vascular Dementia based on Glutamate and N-methyl-DAspartate Receptors in Hippocampal Tissue

作　　者：范耀宗 李彬 FAN Yaozong;LI Bin(Shandong University of Traditional Chinese Medicine,Shandong Qingdao 266033,China;Qingdao Traditional Chinese Medicine Hospital,Shandong Qingdao 266033,China)

机构地区：[1]山东中医药大学第一临床医学院,山东青岛266033 [2]青岛市中医医院,山东青岛266033

出　　处：《中医药临床杂志》2024年第11期2159-2162,共4页Clinical Journal of Traditional Chinese Medicine

基　　金：山东省2019-2020年度中医药科技发展计划项目(2019-0593)。

摘　　要：目的:探讨当归芍药散(DSS)通过海马组织谷氨酸、N-甲基-D-天冬氨酸受体(NMDAR)对血管性痴呆(VD)影响的研究。方法:VD大鼠的造模采用的双侧颈动脉永久结扎法,将造模成功大鼠随机分为5组,假手术组、模型组、DSS低剂量组、DSS高剂量组、阳性对照组。大鼠的学习记忆能力通过Morris水迷宫来进行检测;大鼠海马组织神经元受损情况通过HE染色来观察;采用免疫组化方法测定NMDAR、Caspase-3的含量变化。结果:与假手术组比较,模型组逃避潜伏期显著延长(P<0.01),差异具有统计学意义。与模型组比较,DSS低、高剂量组与尼莫地平组逃避潜伏期缩短(P<0.01);与假手术组比较,模型组的NMDAR、Caspase-3明显升高(P<0.01)。DSS低剂量组、DSS高剂量组、尼莫地平组与模型组比较,DSS高剂量组NMDAR、Caspase-3明显降低(P<0.01)。结论:DSS能明显改善VD大鼠的学习记忆能力,可能通过海马组织谷氨酸、NMDAR对VD起治疗作用。Objective:To investigate the effect of Danggui Shaoyao San(DSS)on vascular dementia(VD)via glutamate and N-methyl-D-aspartate receptors(NMDAR)in hippocampal tissue.Methods:VD rats were modeled by bilateral carotid artery permanent ligation.The rats with successful modeling were randomly divided into 5 groups:sham operation group,model group,DSS low-dose group,DSS high-dose group,and positive control group.The learning and memory ability of rats was tested by Morris water maze;the damage of neurons in the hippocampus of rats was observed by HE staining;and the changes in the contents of NMDAR and Caspase-3 were determined by immunohistochemistry.Results:Compared with the sham operation group,the escape latency of the model group was significantly prolonged(P<0.01),and the difference was statistically significant.Compared with the model group,the escape latency of the low-dose DSS group,high-dose DSS group and nimodipine group was shortened(P<0.01);compared with the sham operation group,the NMDAR and Caspase-3 of the model group were significantly increased(P<0.01).Compared with the model group,the NMDAR and Caspase-3 of the high-dose DSS group were significantly decreased in the lowdose DSS group,high-dose DSS group and nimodipine group(P<0.01).Conclusion:DSS can significantly improve the learning and memory ability of VD rats,and may have a therapeutic effect on VD through hippocampal glutamate and NMDAR.

关 键 词：当归芍药散 血管性痴呆 海马组织谷氨酸 N-甲基-D-天冬氨酸受体 

分 类 号：R285.5[医药卫生—中药学]