40个木槿品种的表型性状多样性及ISSR遗传多样性分析  

Analysis of Phenotypic Trait Diversity and ISSR Genetic Diversity in 40 Hibiscus syriacus Varieties

作　　者：赵文静 闫红民 郑旭 王晓晓 刘兴满[1] 缪美华[1] ZHAO Wenjing;YAN Hongmin;ZHENG Xu;WANG Xiaoxiao;LIU Xingman;MIAO Meihua(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,Jiangsu;Jiangsu Fangyang Construction Engineering Management Co.,Ltd,Lianyungang 222000,Jiangsu)

机构地区：[1]连云港市农业科学院,江苏连云港222000 [2]江苏方洋建设工程管理有限公司,江苏连云港222000

出　　处：《温带林业研究》2024年第4期62-69,共8页Journal of Temperate Forestry Research

基　　金：连云港市财政(CA202210);连云港市财政专项(QNJJ2007);江苏省林业科技创新与推广项目(LYKJ-连云港【2021】01)。

摘　　要：【目的】本研究旨在探究不同木槿品种之间的遗传多样性,为今后木槿的品种培育和改良提供理论支持。【方法】通过表型性状的研究和ISSR分子标记技术对40个木槿品种进行了遗传多样性分析。【结果】表型性状分析结果显示,多样性指数(H')超过1的有20个,说明本研究的供试品种多样性较高,且多集中在花色、花型、花心和花朵及叶片的大小上。数量性状的聚类分析显示,聚类标准侧重于花径和花柱的长度;质量性状的聚类分析结果显示,聚类标准侧重于瓣型和花色;ISSR结果显示,聚类的标准比较倾向于花柱的长度和瓣型。【结论】本研究从表型性状和分子水平两个方面研究了40个木槿品种的遗传多样性,通过表型性状和ISSR技术的遗传多样性分析,我们发现花的瓣型、花柱的长短、花色、花径是木槿品种变异的主要方向,这为木槿以后的品种培育和改良提供了理论依据。【Objective】To explore the genetic diversity among different Hibiscus syriacus varieties and provide theoretical support for the cultivation and improvement of Hibiscus syriacus varieties in the future.【Method】This experiment conducted genetic diversity analysis on 40 Hibiscus syriacus varieties through the study of phenotypic traits and ISSR molecular marker technology.【Result】The analysis of phenotypic traits showed that there were 20 varieties with a diversity index(H')exceeding 1,indicating that the diversity of the tested varieties in this study was relatively high,mainly concentrated in flower color,flower type,flower center,flower and leaf size.Cluster analysis of quantitative traits shows that clustering criteria focus on flower diameter and style length.The clustering analysis results of quality traits show that the clustering criteria focus on petal shape and flower color.The ISSR results show that the clustering criteria tend to favor the length and petal shape of the style.【Conclusion】This study investigated the genetic diversity of 40 Hibiscus syriacus varieties from two aspects:phenotypic traits and molecular level.Through genetic diversity analysis using phenotypic traits and ISSR technology,we found that petal type,style length,color,and diameter of flowers are the main directions of variation in Hibiscus syriacus varieties.This provides a theoretical basis for the cultivation and improvement of Hibiscus syriacus varieties in the future.

关 键 词：木槿 遗传多样性 表型性状 ISSR-PCR 

分 类 号：S685.99[农业科学—观赏园艺]