添加褪黑素对常温和冷冻保存獭兔精液的影响  

Effects of Supplementing Melatonin on Semen Qualities of Rex Bucks under Room Temperature and Cryopreservation Conditions

作　　者：牛晓艳 付海源 詹海杰 李超杰 唐耀平 闫益波 NIU Xiaoyan;FU Haiyuan;ZHAN Haijie;LI Chaojie;TANG Yaoping;YAN Yibo(College of Animal Science,Shanxi Agricultural University,Taigu 030801,China;Guangling Husbandry and Veterinary Service Center,Guangling 037500,China)

机构地区：[1]山西农业大学动物科学学院,太谷030801 [2]广灵县畜牧兽医服务中心,广灵037500

出　　处：《经济动物学报》2024年第4期319-325,共7页Journal of Economic Animal

基　　金：山西省省筹出国留学人员科研项目(2020-157);山西省重点实验室开放课题基金项目(K272107080)。

摘　　要：褪黑素是哺乳动物松果体分泌的一种吲哚类激素,具有较强的抗氧化能力,研究褪黑素在精液保存液中的适宜添加浓度对精液的保存、运输和人工授精具有重要意义。试验采集20只獭兔公兔的精液,在常温(20℃)和冷冻(-196℃)条件下,在精液保存液中添加不同浓度(0,0.05,0.1,0.2,0.4 mmol/L)褪黑素后,观察其在常温下各时间点(12,24,36,48,60,72,96 h)和解冻前后的精子活力、精子密度、精子畸形率。结果表明:常温下添加不同浓度的褪黑素对精子活力、精子密度和精子畸形率无显著影响;冷冻条件下添加褪黑素可显著提高精子活力(P<0.05),其中,在冷冻保存液中添加0.05 mmol/L褪黑素效果较好。Melatonin is an indoles secreted by pineal gland of mammals,which has high antioxidant effects.The study of its best concentration in semen extenders is beneficial for semen preservation,transportation and artificial insemination.In this study,20 Rex bucks were used and their semen samples were collected.After supplementing gradient amounts of melatonin(0,0.05,0.1,0.2,0.4 mmol/L),the sperm motility,density and deformity of all semen samples were observed at different time points(12,24,36,48,60,72,96 h)at room temperature,and after freezing/thawing processes.The results showed that the supplementation of melatonin under room temperature had no significant effects on sperm quality index,while melatonin significantly improved sperm motility(P<0.05)during freezing/thawing process.The best concentration for melatonin supplementation in sperm freezing extender of Rex buck is 0.05 mmol/L.

关 键 词：獭兔 褪黑素 精液 冷冻保存 

分 类 号：S829.1[农业科学—畜牧学]