龙胆泻肝丸中木通薄层鉴别方法研究  

Study on the Identification Method of Thin Layer of Akebia Stem in Longdan Xiegan Pills

作　　者：黄莉 谢婷 李雨 李云 周云峰 高慧 黄招光 HUANG Li;XIE Ting;LI Yu;LI Yun;ZHOU Yunfeng;GAO Hui;HUANG Zhaoguang(Department of Clinical Laboratory,The Second Affiliated Hospital of Yichun University,Jiangxi Province,Yichun 336000,China;Teaching Office,Yichun People's Hospital,Jiangxi Province,Yichun 336000,China;Department of Orthopedics,Yichun Hospital of Traditional Chinese Medicine,Jiangxi Province,Yichun 336000,China;Institute of Drug Control,Yichun Inspection and Testing Center,Jiangxi Province,Yichun 336000,China)

机构地区：[1]宜春学院第二附属医院检验科,江西宜春336000 [2]宜春市人民医院教学办,江西宜春336000 [3]宜春市中医院关节骨科,江西宜春336000 [4]宜春市检验检测中心药品检验所,江西宜春336000

出　　处：《光明中医》2024年第21期4308-4310,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

摘　　要：目的建立龙胆泻肝丸中木通的薄层鉴别法,以期控制该制剂中木通的质量。方法以木通苯乙醇苷B为定性指标,采用硅胶G薄层板,以三氯甲烷-甲醇-水(30∶10∶1)为展开剂,晾干喷以2%香草醛硫酸溶液,在105℃加热数分钟。结果置日光下检视,样品薄层色谱在与木通苯乙醇苷B对照品和木通对照药材色谱相应的位置上均出现相同的红色斑点,而川木通、关木通、甘木通和阴性样品均未有该红色斑点出现,说明所建TLC鉴别法可有效对木通进行定性鉴别。结论所建方法准确、检测成本低,有助于控制该制剂中木通的质量。Objective In order to establish a thin layer identification method for akebia stem in Longdan Xiegan pills to control the quality of mutong of this preparation.Methods The test conditions were based on calceorioside B as the qualitative index,silica gel G thin layer plate,trichloromethane-methanol water(30∶10∶1)as the development agent,sprayed with 2%vanillin sulfuric acid and heated at 105℃for several minutes.Results Under sunlight examination,the same red spots appeared on the TLC of the samples in the corresponding positions with the control substance of calceorioside B and the control medicinal material of mutong.However,the red spots did not appear in the samples of armand clematis stem,manchurian dutchmanspipe stem,clematis filamentosa dunn and the negative samples,indicating that the established TLC identification method can effectively identify the qualitative identification of akebia stem.Conclusion The established method is accurate and low in detection cost,which is helpful to control the quality of akebia stem in the preparation.

关 键 词：龙胆泻肝丸 木通 薄层鉴别法 质量控制 实验研究 

分 类 号：R286.0[医药卫生—中药学]