UPLC-QTOF-MS和GC-MS法表征姜黄提取物中的化学成分及其质量控制  

Characterization and Quality Control of Chemical Composition in Turmeric Extract by UPLC-QTOF-MS and GC-MS

作　　者：林珊 吴彤[1] 李菲 姜国志 LIN Shan;WU Tong;LI Fei;JIANG Guozhi(National Key Lab.of Lead Druggability Research,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 201203;Shenwei Pharmaceutical Group Co.,Ltd.,Shijiazhuang 051430)

机构地区：[1]中国医药工业研究总院,上海医药工业研究院有限公司,先导物成药性研究全国重点实验室,上海201203 [2]神威药业集团有限公司,河北石家庄051430

出　　处：《中国医药工业杂志》2024年第11期1526-1538,共13页Chinese Journal of Pharmaceuticals

基　　金：河北省经典名方与现代中药质量过程控制重点实验室项目(236790057H)。

摘　　要：采用超高效液相色谱-飞行时间质谱(UPLC-QTOF-MS)及气相色谱-质谱(GC-MS)联用法对姜黄提取物进行系统的化学成分表征,并依据ChP 2020年版一部采用UV和HPLC法对其中主要活性成分姜黄素类化合物进行全面质量评价。结果显示,UPLC-QTOF-MS法共鉴别出44种化合物,其中28个姜黄类、10个倍半萜、6个其他类;GC-MS法鉴别出9种挥发性成分。10批姜黄提取物的定量分析结果表明,姜黄素类化合物是其中的主要成分,含量约65%。本研究较全面地反映了姜黄提取物的化学物质基础,为其相关制剂质量控制与临床合理应用提供了数据参考。The ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)and gas chromatography-mass spectrometry(GC-MS)methods were applied to systematically characterize the chemical constituents of turmeric extract.Based on Chinese Pharmacopoeia(2020 edition)VolumeⅠ,the quality control of curcuminoids in turmeric extracts was performed by UV and HPLC methods.The results showed that 44 components(including 28 curcuminoids,10 sesquiterpenes and 6 others)and 9 volatiles were identified by UPLC-QTOF MS and GC-MS,respectively.The results of quantitative analysis indicated curcumins were main components in extracts,which accounted for 65%among 10 batches.The significance of this study was to comprehensively reveal the chemical constituents of turmeric extract,which provided essential data for quality control and clinical application of the pharmaceutical dosage forms of turmeric extracts.

关 键 词：姜黄提取物 UPLC-QTOF-MS GC-MS 化学成分 表征 鉴定 姜黄素 倍半萜 质量评价 

分 类 号：O657.7[理学—分析化学] O657.63[理学—化学] R284.1[医药卫生—中药学]