杭州市2023年污水环境中霍乱弧菌的毒力及耐药特征  

Virulence and antibiotic resistance characteristics of Vibrio cholerae in the sewage environment of Hangzhou in 2023

作　　者：王凌波 石国祥[1] 吴蓓蓓[1] 姚文武 吴卓颖 吴亦斐 杨章女[1] WANG Lingbo;SHI Guoxiang;WU Beibei;YAO Wenwu;WU Zhuoying;WU Yifei;YANG Zhangnyu(Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China;Hangzhou Center for Disease Control and Prevention,Hangzhou Institute of Health Supervision,Hangzhou,Zhejiang 310028,China)

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051 [2]杭州市疾病预防控制中心,杭州市卫生监督所,浙江杭州310028

出　　处：《中国热带医学》2024年第11期1306-1311,1319,共7页China Tropical Medicine

基　　金：国家重点研发计划项目(No.2023YFC2605100,No.2023YFC2605102);浙江省自然科学基金项目(No.LFG20H260003);浙江省医药卫生科技计划项目(No.2023ZL352;2020KY238)。

摘　　要：目的对杭州市城市污水环境病原菌进行分离和鉴定,了解污水分离霍乱弧菌的血清型、耐药特征并预测毒力基因、耐药基因携带情况。方法于2023年1—10月连续采集杭州市不同环境城市污水样本进行病原菌分离培养,对分离的27株霍乱弧菌以玻片凝集法进行血清型鉴定,使用微量肉汤稀释法进行药物敏感性实验,通过全基因组序列测定,运用生物信息学软件对耐药基因和毒力基因进行预测。结果污水环境病原菌进行分离和鉴定,显示包括沙门菌属、弧菌属、肠杆菌属、气单胞菌属等26个菌属82种菌种,其中27株霍乱弧菌均为非O_(1)非O_(139)血清群,对氨苄西林、氨苄西林/舒巴坦和复方新诺明的耐药率较高,分别为59.26%、14.81%、14.81%,对头孢噻肟和四环素、氯霉素、美罗培南、厄他培南、头孢他啶、头孢他啶/阿维巴坦、阿米卡星、环丙沙星、替加环素、阿奇霉素均表现高敏感性;对其中11株霍乱弧菌共检测出21种耐药基因,其中β-内酰胺类耐药基因的携带率最高达63.64%,其中有1株菌株携带17种耐药基因,11株霍乱弧菌均不携带ctxA、ctxB、ace、zot、chxA基因、所有菌株均携带rtxB、rtxC、rtxD基因,90.91%的菌株携带hlyA,36.36%的菌株携带rtxA。结论本研究污水分离霍乱弧菌均为非O_(1)非O_(139)血清群,对大多数抗菌药物表现出较好的敏感性,也携带一定数量的耐药基因与毒力基因,需重点关注。Objective To analyze the identification of pathogenic bacteria in municipal sewage of Hangzhou City,Zhejiang Province from January to October 2023,to understand the serotype and drug resistance of Vibrio cholerae isolated from sewage,as well as to predict the virulence gene and drug resistance gene carrying situation.Methods From January to October 2023,urban sewage samples from various environments in Hangzhou City,Zhejiang Province,were continuously collected for bacterial isolation and culture.The serotypes of 27 Vibrio cholerae strains were identified by slide agglutination method,and the drug sensitivity test was conducted by micro broth dilution method.Whole genome sequence and bioinformatics software were used to predict drug resistance genes and virulence genes.Results The sewage identification results showed 26 bacteria genera,including Salmonella,Vibrio,Enterobacter,and Aeromonas,among 82 bacterial species.All 27 strains of Vibrio cholerae were non-O_(1) and non-O_(139) serogroups.The strains showed high resistance rates to ampicillin,ampicillin/sulbactam and cotrimoxazole at 59.26%,14.81%,and 14.81%,respectively),exhibited high sensitivity to cefotaxime,tetracycline,chloramphenicol,meropenem,ertapenem,ceftazidime,ceftazidime/avibactam,amikacin,ciprofloxacin,tigecycline,and azithromycin.A total of 21 different drug resistance genes were detected in 11 strains of Vibrio cholerae,among which the highest carriage rate ofβ-lactam resistance genes reached 63.64%,and 1 strain carried 17 kinds of drug resistance genes.None of the 11 strains of Vibrio cholerae carried ctxA,ctxB,ace,zot,or chxA genes,but all the strains carried rtxB,rtxC,and rtxD genes;90.91%of the strains carried hlyA,and 36.36%carried rtxA.Conclusions All Vibrio cholerae isolated from sewage in this study are non-O_(1) and non-O_(139) serogroups,showing good sensitivity to most antibiotics,but also they carry a certain number of drug-resistance genes and virulence genes.This suggests that future monitoring efforts should focus on these strain

关 键 词：污水监测 霍乱弧菌 耐药特征 毒力基因 

分 类 号：R378.3[医药卫生—病原生物学]