2022-2023年百色地区HIV-1主要流行毒株研究  

Major HIV-1 epidemic strains in the Baise region from 2022 to 2023

作　　者：田红玉 陈赞[1] 陈坚[1] 黄安恒 汤健闻[1] 罗大泉[1] 韦忠旺 蓝光华[2] 李剑军[2] TIAN Hongyu;CHEN Zan;CHEN Jian;HUANG Anheng;TANG Jianwen;LUO Daquan;WEI Zhongwang;LAN Guanghua;LI Jianjun(Baise Center for Disease Prevention and Control,Baise 533000,Guangxi,China;Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control,Nanning 530028,Guangxi,China)

机构地区：[1]百色市疾病预防控制中心,广西百色533000 [2]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《中国艾滋病性病》2024年第11期1168-1172,共5页Chinese Journal of Aids & STD

基　　金：广西重大传染病防控与生物安全应急响应重点实验室开放课题(GKLKP-KF-202205);广西重大传染病防控与生物安全应急响应重点实验室(21-220-12);国家科技部重点研发专项(2022YFC2305201);广西自然科学基金(2020GXNSFAA159020)。

摘　　要：目的分析百色市辖靖西市、那坡县、德保县和右江区(简称百色地区)HIV-1主要流行毒株及特征。方法以2022-2023年成人新发现报告HIV/AIDS患者为研究对象,对其血浆进行病毒核酸提取、反转录扩增、测序、HIV-1基因亚型和亚簇判定。结果共纳入分析257例新发现报告HIV/AIDS患者,其中,≥50岁中老年人占56.0%,男性占65.0%,农民占85.2%,壮族占84.0%,文盲及小学占51.4%,已婚有配偶占56.8%,异性性传播占93.0%。共发现8种基因亚型,其中,CRF08_BC(51.0%,131例)、CRF01_AE(31.1%,80例)和CRF07_BC(12.8%,33例)为3个主要流行毒株。不同年龄段、人群分类、感染途径和现住址之间的基因亚型分布差异有统计学意义(P均<0.05)。3个主要毒株聚集成6个较大亚簇和12个较小亚簇,其中,以右江区序列为主聚集成3个较大亚簇(CRF08_BC亚簇1、CRF08_BC亚簇5,CRF01_AE新亚簇3),以靖西市序列为主聚集成1个较大亚簇(CRF08_BC亚簇8),以德保县序列为主聚集成1个较大亚簇(CRF01_AE新亚簇1),1个较大亚簇(CRF07_BC亚簇2)未呈县区聚集性。结论2022-2023年中越边境百色地区流行毒株呈复杂性和多样性,但仍以CRF08_BC、CRF01_AE和CRF07_BC为3个主要流行毒株且聚集成多个明显亚簇,亚簇呈县区聚集性。Objective To analyze the predominant HIV-1 strains and their characteristics in the Baise region,encompassing Jingxi City,Napo County,Debao County,and Youjiang District.Methods Participants were selected from newly reported adult patients infected with HIV between 2022 and 2023,and their plasma samples were collected for viral RNA extraction,reverse transcription amplification,sequencing,and determination of HIV-1 subtype and subcluster.Results A total of 257 newly reported HIV/AIDS cases were analyzed.Among these,56.0%were aged 50 years and above,65.0%were male,85.2%were farmers,and 84.0%were of Zhuang ethnicity.Illiteracy or primary school education was reported by 51.4%of the patients,and 56.8%were married.Heterosexual transmission accounted for 93.0%of the cases.Eight genetic subtypes were identified,with CRF08_BC(51.0%,131 cases),CRF01_AE(31.1%,80 cases),and CRF07_BC(12.8%,33 cases)being the predominant strains.The distribution of these subtypes varied significantly across age groups,demographics,transmission routes,and residential areas(all P<0.05).The three main strains clustered into six larger subclusters and 12 smaller subclusters.Sequences from Youjiang District predominantly clustered into three major subclusters(CRF08_BC subclusters 1 and 5,and a new CRF01_AE subcluster 3).The Jingxi City sequences formed one major subcluster(CRF08_BC subcluster 8),while the Debao County sequences formed one major subcluster(a new CRF01_AE subcluster 1).One large subcluster(CRF07_BC subcluster 2)exhibited no regional clustering.Conclusions HIV-1 epidemic strains in the ChinaVietnam border area of Baise from 2022 to 2023 exhibited complexity and diversity.However,CRF08_BC,CRF01_AE,and CRF07_BC remained the predominant strains and clustered into multiple distinct subclusters with evident regional characteristics.

关 键 词：艾滋病病毒 基因亚型 亚簇 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]