益肾痛泄方对腹泻型肠易激综合征大鼠IL-2、IL-10、TGF-β、IFN-γ表达的影响  

Effects of Yishen Tongxie Formula on the Expression of IL-2,IL-10,TGF-βand IFN-γin Rats with Diarrhea-type Irritable Bowel Syndrome

作　　者：陈映西 谢胜[2] 黄晓燕[2] 郑超伟[2] 王萌[2] 韦金秀 陈广文[2] 李建锋 王道刚[2] CHEN Ying-xi;XIE Sheng;HUANG Xiao-yan;ZHENG Chao-wei;WANG Meng;WEI Jin-xiu;CHEN Guang-wen;LI Jian-feng;WANG Dao-gang(Guilin Municipal Hospital of Traditional Chinese Medicine,Guilin,Guangxi,541000;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning,Guangxi,530023)

机构地区：[1]桂林市中医医院,广西桂林541000 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《广西中医药大学学报》2024年第6期38-43,64,共7页Journal of Guangxi University Of Chinese Medicine

基　　金：广西科技基地和人才专项课题(编号:桂科AD19245168);广西中医药大学校级科研项目(编号:2019MS016)。

摘　　要：[目的]研究益肾痛泄方对腹泻型肠易激综合征模型大鼠小肠组织白细胞介素2(IL-2)、白细胞介素10(IL-10)、转化生长因子β(TGF-β)、γ-干扰素(IFN-γ)表达水平的影响。[方法]将24只Wistar大鼠按随机数字表法分为正常组、模型组、奥替溴铵组、益肾痛泄方组,每组6只,除正常组大鼠外,其余各组均采用4%醋酸灌肠叠加慢性心理应激法复制腹泻型肠易激综合征模型,奥替溴铵组、益肾痛泄方组大鼠连续给药14 d。观察大鼠的一般情况,大鼠处死后,采用苏木素-伊红(HE)染色法观察各组大鼠小肠组织病理变化,采用实时荧光定量聚合酶链式反应(RT-PCR)及蛋白免疫印迹法(Western Blot)分别检测各组大鼠小肠组织IL-2、IL-10、TGF-β、IFN-γmRNA及蛋白表达水平。[结果]与正常组比较,模型组大鼠小肠出现明显的淋巴细胞灶性浸润,且模型组大鼠小肠组织中IL-2、IL-10、TGF-β、IFN-γmRNA及蛋白表达水平均显著升高,差异有统计学意义(P<0.01);与模型组比较,奥替溴铵组、益肾痛泄方组大鼠小肠组织未见明显炎症浸润,且小肠IL-2、IL-10、TGF-β、IFN-γmRNA及蛋白表达水平均显著降低(P<0.05或P<0.01);与奥替溴铵组比较,益肾痛泄方组大鼠小肠组织固有层肠腺丰富,未见明显炎症细胞浸润,IL-2、IL-10、TGF-β、IFN-γmRNA及蛋白表达水平均低于奥替溴铵组,但差异无统计学意义(P>0.05)。[结论]益肾痛泄方可能通过降低IL-2、IL-10、TGF-β、IFN-γ的表达,进而缓解小肠炎症损伤,发挥治疗腹泻型肠易激综合征的作用。[Objective]To study the effects of Yishen Tongxie formula on the expression levels of IL-2,IL-10,TGF-βand IFN-γin the small intestine of rats with diarrheal irritable bowel syndrome[.Methods]Twenty-four wistar rats were divided into normal group,model group,Otilonium bromide group and Yishen Tongxie formula group by random number table method,with 6 rats in each group.By using 4%acetic acid enemas combined with chronic psychological stress,the diarrhea-type irritable bowel syndrome model was built in all groups except the normal group.In the Otilonium bromide group and Yishen Tongxie formula group,the related drugs were administered for 14 days after the model was built.The general condition of the rats was observed before execution.HE staining was used to observe the pathological changes of small intestine,and RT-PCR and Western blot were used to measure the mRNA and protein expression levels of IL-2,IL-10,TGF-βand IFN-γin small intestine.[Results]Compared with the normal group,obvious focal lymphocyte infiltration in the small intestine of the rats in the model group was observed,and the mRNA and protein expression levels of IL-2,IL-10,TGF-βand IFN-γin the small intestine of the rats in the model group were significantly increased(P<0.01).Compared with the model group,no obvious inflammatory infiltration in small intestinal tissues was observed in Otilonium bromide group and Yishen Tongxie formula group,and the mRNA and protein expression levels of IL-2,IL-10,TGF-βand IFN-γwere significantly decreased in Otilonium bromide group and Yishen Tongxie formula group(P<0.05 or P<0.01).Compared with Otilonium bromide group,abundant intestinal glands in the lamina propria of small intestine and no obvious inflammatory cell infiltration were observed in Yishen Tongxie formula group,and the mRNA and protein expressions of IL-2,IL-10,TGF-βand IFN-γwere lower than those of Otilonium bromide group,with no statistical significance(P>0.05)[.Conclusion]Yishen Tongxie formula may alleviate the damage of intestinal infl

关 键 词：益肾痛泄方 腹泻型肠易激综合征 肠道炎症反应 IL-2 IL-10 TGF-Β IFN-Γ 实验研究 

分 类 号：R285.5[医药卫生—中药学]