非运动型红球菌OS62-1在紫花苜蓿根系吸附及迁移  

Adsorption and migration of non-motile Rhodococcus sp.OS62-1 in alfalfa roots

作　　者：柳晓东 刘鑫慧 姜影影 王佳敏 LIU Xiaodong;LIU Xinhui;JIANG Yingying;WANG Jiamin(School of Life Sciences,Yan’an University,Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau,Yan’an 716000,China)

机构地区：[1]延安大学生命科学学院,陕西省黄土高原资源植物研究与利用省市共建重点实验室,陕西延安716000

出　　处：《延安大学学报（自然科学版）》2024年第4期7-13,共7页Journal of Yan'an University：Natural Science Edition

基　　金：国家自然科学基金项目(42207037);延安市科技专项经费项目(2019-27,203010105);延安大学校级大学生创新创业训练计划项目(D2022013)。

摘　　要：探究非运动型红球菌OS62-1(Rhodococcus sp.OS62-1)在紫花苜蓿根系吸附及迁移情况。采用扫描电镜及梯度稀释涂平板方法观察并统计OS62-1在紫花苜蓿根系的吸附情况;利用梯度稀释涂平板的方法研究OS62-1在不同土壤及联合修复体系中的迁移情况;利用红外测油仪对不同深度石油污染土壤的残留石油浓度进行测定,探究不同修复方式对不同深度石油污染土壤的修复效果。结果显示,菌株OS62-1能够有效吸附到紫花苜蓿根系,菌落计数统计结果表明紫花苜蓿根系对OS62-1的吸附数量约为(7.7±1.0)×10^(3) CFU/mm^(2)(Colony forming units)。菌株OS62-1在未污染土壤中迁移能力远高于石油污染土壤,在未污染土壤中OS62-1在第7 d时已迁移到6 cm深度,而在石油污染土壤中仅迁移到2 cm深度。在污染土壤中紫花苜蓿根际检测到菌株OS62-1,但是在根系未达到的深度没有检测的OS62-1。经过11 d的修复,单接菌及联合修复处理石油污染土残留石油浓度都显著低于对照组,并且处理组各深度土壤石油残留浓度都低于对照组。非运动型红球菌OS62-1能够吸附到紫花苜蓿根系并随之进行迁移,进而提高联合修复体系石油污染修复效率。To explore the adsorption and migration of non-motile Rhodococcus sp.OS62-1 with alfalfa roots.The adsorption of OS62-1 on alfalfa roots was observed and counted by scanning electron microscopy and gradient dilution plate method.The migration of OS62-1 in different soils and combined remediation systems was studied by gradient dilution plate method.The residual oil concentration of petroleum contaminated soil at different depths was measured by infrared oil meter,and the remediation effect of different remediation methods at different depths was explored.The results showed that strain OS62-1 was able to effectively adsorb to the alfalfa root system,and the number of OS62-1 cells adsorbed to alfalfa roots was approximately(7.7±1.0)×10^(3) CFU/mm^(2)(colony forming units).The migration ability of strain OS62-1 in uncontaminated soil was much higher than that in petroleum contaminated soil.On the 7th day,OS62-1 had migrated to the depth of 6 cm in uncontaminated soil,while it only migrated to the depth of 2 cm in petroleum contaminated soil.Strain OS62-1 was detected in the rhizosphere of alfalfa in contaminated soil,but OS62-1 was not detected at the depth not reached by the roots.After 11 days repair,the residual oil concentration of oil-contaminated soil treated by single inoculation of OS62-1 and combined remediation was significantly lower than that in the control group,and the residual oil concentration of soil at all depths in the treatment group was lower than that in the control group.The non-motile Rhodococcus sp.OS62-1 can adsorb and migrate to alfalfa root system,thus improving the efficiency of oil pollution remediation in the combined remediation system.

关 键 词：非运动型细菌 迁移 石油污染 微生物修复 吸附 紫花苜蓿 

分 类 号：Q93[生物学—微生物学]