复合益生菌对小鼠的安全性研究  

Safety study on compound probiotics in mice

作　　者：张思雨 武亚楠 孙杭 赵伊阳 黄莉莉[1] ZHANG Siyu;WU Yanan;SUN Hang;ZHAO Yiyang;HUANG Lili(School of Pharmacy,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中国乳品工业》2024年第11期25-30,共6页China Dairy Industry

基　　金：黑龙江省博士后科研启动金(LBH-Q13161);黑龙江省自然科学基金(LH2019H106);黑龙江中医药中青年科技攻关项目(ZQG-042)。

摘　　要：文章对动物双歧杆菌乳亚种XLTG11、干酪乳酪杆菌LP16、鼠李糖乳酪杆菌GloryLG12和植物乳植杆菌CCFM8661组成的复合益生菌进行安全性评价。采用《实验动物微生物、寄生虫学等级及监测》《生活饮用水卫生标准》《实验动物配合饲料通用质量标准》等国家标准方法,每周记录大鼠体质量、摄食及饮水摄入量,对大鼠进行眼部、血液学、血生化、尿液检查及病理检查。结果表明,复合益生菌在试验剂量下,各组试验结果之间无无统计学差异,不会引起大鼠各个器官病理学变化与血液生理指标异常。因此复合菌具有生物安全性。The article evaluates the safety of a composite probiotic consisting of four species:Bifidobacterium lactis subsp.XLTG11,Lactobacllus casei LP16,Lactobacillus rhamnosus Glory LG12,and Plantarum lactis CCFM8661.Using national standard methods such as"Microbial and Parasitic Grades and Monitoring of Experimental Animals","Sanitary Standards for Drinking Water",and"General Quality Standards for Compound Feed of Experimental Animals",the body weight,food intake,and water intake of rats were recorded weekly.Eye,hematological,biochemical,urine,and pathological examinations were performed on rats.The results showed that there was no statistically significant difference in the experimental results between the groups of composite probiotics at the experimental dose,and it did not cause pathological changes in various organs and abnormal blood physiological indicators in rats.It can be inferred that the composite bacteria have biological safety.

关 键 词：动物双歧杆菌乳亚种 干酪乳酪杆菌 鼠李糖乳酪杆菌 植物乳植杆菌 复合益生菌 生物安全性 

分 类 号：TS252.42[轻工技术与工程—农产品加工及贮藏工程]