超高效液相色谱串联质谱法快速检测液态乳中41种磺胺及喹诺酮类化合物  

Rapid determination of 41 sulfonamides and quinolones antibiotics in liquid milk by QuEChERS purification and liquid chromatography tandem mass spectrometry with Scheduled MRM mode

作　　者：黄珊[1] 欧阳小艳 范云燕[1] HUANG Shan;OUYANG Xiaoyan;FAN Yunyan(Nanning Center for Disease Control and Prevention,Nanning 530023)

机构地区：[1]南宁市疾病预防控制中心,南宁530023

出　　处：《中国乳品工业》2024年第11期67-74,共8页China Dairy Industry

基　　金：南宁市科技局重点研发计划项目(ZC20223240)。

摘　　要：文章建立了使用C18粉末分散固相萃取净化,超高效液相色谱串联三重四级杆质谱仪快速检测液态乳中41种磺胺及喹诺酮类化合物的方法。样品用2%的甲酸乙腈提取,离心后取上清液用C18粉末净化,净化后的样品稀释一倍后用超高效液相色串联三重四级杆质谱仪在Scheduled MRM模式下检测,以基质匹配的标准曲线定量。结果表明,41种磺胺及喹诺酮化合物在检测范围内线性关系良好,各化合物标准曲线相关系数r≥0.998,样品加标回收率为78%~116%,RSD为0.5%~9.9%,检出限范围0.05~0.20μg/kg,定量限范围0.15~0.60μg/kg。该方法快速、简单、灵敏度高、稳定性好、检测成本低,适用于液态乳中41种磺胺及喹诺酮类化合物快速检测。In this study,a method was developed for the rapid detection of 41 sulfonamides and quinolones in liquid milk by ultra-performance liquid chromatography tandem triple quadrupole-mass spectrometry(UPLC-MS/MS)using C18 powder purification.Sulfonamidesand and quinolones were extracted from milk with 2%formic acid acetonitrile,followed by centrifuging,purifying and diluting,at last,the sample was analysed with UPLC-MS/MS on Scheduled MRM mode,quantitative by matrix matching standard curve.The results showed that 41 sulfonamides and quinolones had good linearity in the detection range,and the correlation coeficient of each compound r≥0.998.The average recovery of the method was in the range of 78%-116%,with the relative standard deviation of 0.5%-9.9%.The limits of detection range were 0.05-0.20μg/kg,limits of quantification range were 0.15-0.60μg/kg.The method had the characteristics of easy operation,fast speed,high accuracy,good repeatability,high sensitivity and low cost.It is suitable for the rapid detection of 41 sulfonamides and quinolones in liquid milk.

关 键 词：超高效液相色谱串联三重四级杆质谱仪 C18 快速检测 磺胺 喹诺酮 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]