提高双肌臀夏洛来羊胚胎移植效果的研究  

Studies to improve the effect of embryo transfer in callipyge Charolais sheep

作　　者：王国春 夏德翠 王泽英[3] 杜学海 杨术环 Wang Guochun;Xia Decui;Wang Zeying;Du Xuehai;Yang Shuhuan(Liaoning Agricultural Development Service Centre,Liaoning Shenyang 110032;Animal Disease Prevention and Control Centre of Qinghemen District,Fuxin City,Liaoning Fuxin 123006;College of Animal Science and Medicine,Shenyang Agricultural University,Liaoning Shenyang 110866)

机构地区：[1]辽宁省农业发展服务中心,辽宁沈阳110032 [2]阜新市清河门区动物疫病预防控制中心,辽宁阜新123006 [3]沈阳农业大学动物科学与医学学院,辽宁沈阳110866

出　　处：《现代畜牧兽医》2024年第10期7-11,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：2023年辽宁省“揭榜挂帅”科技计划项目(技术攻关类)“夏洛来肉羊新品系选育(2023JH1/10400023)”。

摘　　要：研究旨在确定双肌臀夏洛来羊母羊超数排卵时使用促卵泡激素(FSH)的最佳剂量和最佳膘情体况以及最佳胚胎移植月份,为双肌臀夏洛来羊胚胎移植方案优化提供参考。试验对比不同剂量FSH进行超数排卵、不同月份进行胚胎移植的双肌臀夏洛来羊母羊的出胚数、受胎率,对比不同膘情体况的双肌臀夏洛来羊母羊的出胚数。结果显示,不同年龄双肌臀夏洛来羊母羊超数排卵最佳FSH剂量及注射方法为:周岁母羊使用FSH总量为140 IU,以25 IU为首次量逐日递减;2~5岁母羊使用FSH总量为160 IU,以28 IU为首次量逐日递减;6岁以上母羊使用FSH总量为180 IU,以32 IU为首次量逐日递减。辽宁地区双肌臀夏洛来羊最佳胚胎移植月份是10~12月,受胎率、胚胎可用率最高。双肌臀夏洛来羊母羊最佳超数排卵膘情是通过短期优饲将供体母羊从6.5成膘情提高到8成膘情,平均只出胚数为11.4枚。研究表明,在辽宁地区的10~12月,以2~5岁的经产双肌臀夏洛来羊母羊为供体,进行短期优饲将膘情从6.5成提高到8成后,FSH以28 IU为首次量逐日递减,胚胎移植效果最为理想。The aim of this study was to determine the optimal dose of follicle-stimulating hormone(FSH)and the best fatness and the best embryo transfer month in ewes with superovulation,and to provide a reference for the optimization of embryo transfer scheme in callipyge Charolais sheep.The experiment compared the embryo number and fertilization rate of ewes of callipyge Charolais sheep with different doses of FSH and embryo transfer in different months,and compared the embryo number of ewes of callipyge Charolais sheep with different fatness.The results showed that the optimal dose and injection method of FSH in ewes with superovulation of callipyge Charolais sheep at different ages were 140 IU in total FSH used by ewes at one year of age,with 25 IU as the first dose decreasing day by day.The total FSH used by ewes aged 2 to 5 years was 160 IU,with 28 IU as the first dose decreasing day by day.The total amount of FSH used by ewes over 6 years old was 180 IU,with 32 IU as the first dose decreasing day by day.The best month for embryo transfer of callipyge Charolais sheep in Liaoning was October to December,and the conception rate and embryo availability rate were the highest.The optimal ovulatory condition of callipyge Charolais sheep ewes was to increase the donor ewes'fatness from 6.5 to 8.0 by short-term optimal feeding,and the average number of embryos was 11.4.The results showed that in October to December in Liaoning province,2 to 5 years old callipyge Charolais sheep ewes were used as donors to improve the body condition from 6.5 to 8.0 in a short term,and the FSH was decreased day by day at 28 IU for the first time,and the embryo transfer effect was the best.

关 键 词：双肌臀夏洛来羊 胚胎移植 超数排卵 膘情 

分 类 号：S814[农业科学—畜牧学]