虾夷扇贝脓疱病病原菌的分离鉴定及复方中草药防治效果研究  

Isolation and identification of pathogenic bacteria from Patinopecten yessoensis and its prevention and treatment with compound Chinese herbal medicine

作　　者：左文婷 戴伟 吴梦瑜 尤宏争[2] 宋伦[3] 毕相东 Zuo Wenting;Dai Wei;Wu Mengyu;You Hongzheng;Song Lun;Bi Xiangdong(Key Laboratory of Aqua-ecology and Aquaculture of Tianjin,College of Fisheries Sciences,Tianjin Agricultural University,Tianjin 300392;Tianjin Fishery Research Institute,Tianjin 300221;Liaoning Ocean and Fisheries Science Research Institute,Liaoning Dalian 116023)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300392 [2]天津市水产研究所,天津300221 [3]辽宁省海洋水产科学研究院,辽宁大连116023

出　　处：《现代畜牧兽医》2024年第10期52-58,共7页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：天津市科技计划项目(24ZYCGSN00140);甘肃省科技计划项目(24CX6NP223);天津市大学生创新训练计划项目(202210061057)。

摘　　要：试验旨在探讨复方中草药防治虾夷扇贝脓疱病的可行性,从患脓疱病虾夷扇贝的病灶部位分离纯化病原菌,通过体外试验检测复方中草药的抑菌效果,并通过人工回感试验检测复方中草药对该病原菌的防治效果。结果显示,经生理生化及分子生物学鉴定,分离病原菌为海藻希瓦氏菌(Shewanella algae)。该菌株对头孢曲松、氨曲南、氧氟沙星等10种药物高度敏感,对头孢西汀和头孢噻吩不敏感。复方中草药对该菌株的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为0.625 g/L和1.25 g/L。饲料中添加复方中草药能够显著降低虾夷扇贝体内细菌量(P<0.05),显著提高虾夷扇贝抗氧化酶活性(P<0.05),降低虾夷扇贝死亡率。研究表明,可采用在饲料中添加复方中草药的方式防治海藻希瓦氏菌诱发的虾夷扇贝脓疱病,具有良好的应用前景。The purpose of this study was to investigate the feasibility of compound Chinese herbal medicine for preventing and treating impetigo of Patinopectenyessoensis.The pathogenic bacteria were isolated and purified from the lesion site of Patinopectenyessoensis.The antibacterial effect of the compound Chinese herbal medicine was detected by invitro test,and the control effect of the compound Chinese herbal medicine on the pathogenic bacteria was detected by artificial response test.The results showed that the pathogen was identified as Shewanellaalgae by physiological,biochemical and molecular biology.The strain was highly sensitive to 10 drugs such as ceftriaxone,amtronam and ofloxacin,but was not sensitive to sporacetine and cefalothiophene.The MIC and MBC of compound Chinese herbal medicine were 0.625 g/L and 1.25 g/L,respectively.The addition of compound Chinese herbal medicine in the diet could significantly reduce the bacteria content in the body of Patinopecten yessoensis(P<0.05),significantly increase the antioxidant enzyme activity of Patinopecten yessoensis(P<0.05),and reduce the mortality of Patinopecten yessoensis.The study showed that the method of adding compound Chinese herbal medicine to the feed can prevent and control the impetigo of Patinopecten yessoensis induced by Shewanella algae,which is a good application prospect.

关 键 词：复方中草药 虾夷扇贝 脓疱病 海藻希瓦氏菌 

分 类 号：S944.43[农业科学—水产养殖]