渭南地区牛羊口蹄疫免疫效果评估及分析  

Evaluation and analysis of immune effect of foot-and-mouth disease in cattle and sheep in Weinan city

作　　者：秦鸽鸽 白鸽 南苹瑶 徐梦迪 王国超 任倩 吴奇强 Qin Gege;Bai Ge;Nan Pingyao;Xu Mengdi;Wang Guochao;Ren Qian;Wu Qiqiang(Weinan Animal Disease Control Center,Shaanxi Weinan 714000)

机构地区：[1]渭南市动物疫病预防控制中心,陕西渭南714000

出　　处：《现代畜牧兽医》2024年第10期63-66,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：陕西省农业科技创新转化项目(NYKJ-2019-WN001)。

摘　　要：为了解和掌握渭南市辖区内牛、羊O型口蹄疫免疫效果及病原流行状况,于2020—2023年对全市O型口蹄疫免疫牛、羊进行抽样调查,采用液相阻断ELISA和荧光RT-PCR检测方法进行检测。结果显示:渭南市牛、羊O型口蹄疫免疫抗体平均阳性率分别为85.72%、75.25%,325份食道-咽部分泌物(OP液)经荧光RTPCR检测均未检出O型口蹄疫病毒;不同年份牛、羊O型口蹄疫免疫抗体阳性率分别为86.16%、75.52%、83.97%、91.77%和74.92%、70.45%、72.94%、80.00%;中小型规模场牛、羊免疫抗体阳性率为89.33%、86.79%,散养户牛、羊免疫抗体阳性率为87.01%、77.73%;不同区域牛、羊O型口蹄疫免疫抗体阳性率分别为70.30%~96.36%和66.36%~87.39%。研究表明,近年来渭南市牛、羊O型口蹄疫免疫效果整体较好,可疑的动物样本均未检出O型口蹄疫病毒,部分区域存在牛、羊免疫效果不佳,应引起高度重视,严格实行强制免疫方案,为科学健康养殖提供保障基础。In order to understand and grasp the immune effect and epidemic status of foot-and-mouth disease serotype O in cattle and sheep under the jurisdiction of Weinan city,samples of foot-and-mouth disease serotype O immune cattle and sheep in the city were investigated from 2020 to 2023,and liquid phase blocking ELISA and fluorescent RT-PCR detection methods were used for detection.The results showed that the average positive rate of foot-and-mouth disease serotype O antibody in cattle and sheep in Weinan city was 85.72%and 75.25%,respectively.No foot-and-mouth disease virus serotype O was detected in 325 coesophagopharyngeal secretions(OP fluid)by fluorescent RT-PCR.The positive rates of FMD O antibody in cattle and sheep in different years were 86.16%,75.52%,83.97%,91.77%,and 74.92%,70.45%,72.94%,80.00%,respectively.The positive rates of immune antibody were 89.33%and 86.79%in small and medium-sized farms,87.01%and 77.73%in free-range farms.The positive rates of foot-and-mouth disease serotype O antibody in cattle and sheep in different regions were 70.30%~96.36%and 66.36%~87.39%,respectively.Studies have shown that in recent years,the immune effect of foot-and-mouth disease serotype O in cattle and sheep in Weinan city is generally good,and no foot-and-mouth disease virus serotype O has been detected in suspicious animal samples,and the immune effect of cattle and sheep in some areas is poor,which should be paid great attention to and the compulsory immunization program should be strictly implemented to provide a guarantee basis for scientific and healthy breeding.

关 键 词：口蹄疫 免疫效果 抗体检测 病原学检测 

分 类 号：S851[农业科学—预防兽医学]