钛/铋氧化物纳米材料用于声动力/放射联合治疗肿瘤  

Titanium/bismuth oxide nanomaterials for sonodynamic/radiation combined therapy of tumors

作　　者：郑之裕 修梦婷 王通 张艳艳 朱利民[1] ZHENG Zhiyu;XIU Mengting;WANG Tong;ZHANG Yanyan;ZHU Limin(College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学生物与医学工程学院,上海201620

出　　处：《精细化工》2024年第12期2668-2673,共6页Fine Chemicals

基　　金：上海市科学技术委员会资助项目(22520710400,21WZ2501300和20DZ2254900);教育部生物医药纺织材料“111项目”(B07024)。

摘　　要：以钛酸四丁酯和冰醋酸为原料,经水热反应制备了TiO_(2)纳米晶体,其与Bi(NO_(3))_(3)反应得到Bi_(2)O_(3)/Ti O_(2)纳米颗粒(BTH),BTH经聚多巴胺(PDA)涂层包覆,得到了BTH@PDA纳米颗粒(BTP)。通过TEM、XRD和XPS对BTP形貌和组成进行了表征,采用生物安全性实验、体外细胞毒性测试、体外细胞摄取和活性氧染色测试了BTP的生物相容性、细胞毒性和活性氧生成能力。结果表明,BTP呈梭形,最长边粒径(125.18±14.66)nm,Zeta电位为(–4.17±0.33)m V;BTP悬液在远高于正常治疗质量浓度(300μg/m L)的条件下,对L929细胞和4T1细胞无明显的毒性;BTP能增强放疗的疗效,在超声和X射线共同处理后,能将4T1细胞的存活率降至34.3%,并能有效产生活性氧;4T1细胞对BTP的摄取具有时间依赖性,在12 h时有最大摄取。TiO_(2)nanocrystals were synthesized from hydrothermal reaction of tetrabutyl titanate and acetic acid,and then reacted with Bi(NO_3)_(3)to prepare Bi_(2)O_(3)/TiO_(2)nanoparticles(BTH),which was further coated with polydopamine(PDA)to obtain BTH@PDA nanoparticles(BTP).The BTP nanoparticles were characterized by TEM,XRD and XPS for analyses on morphology and composition,and evaluated through biosafety test,cytotoxicity assay,cellular uptake assay and reactive oxygen species(ROS)staining,respectively,on their biosafety,biocompatibility and the ability of ROS generation.The results indicated that BTP exhibited a fusiform shape,with the longest side having a particle size of(125.18±14.66)nm,and a Zeta potential of(–4.17±0.33)m V.BTP suspension showed no obvious toxicity towards L929 and 4T1cells at a mass concentration significantly above normal treatment concentration(300μg/m L).Meanwhile,BTP could enhance the therapeutic outcome of radiotherapy and effectively generate ROS in response to ultrasound.After co-treatment by ultrasound and X ray,BTP could reduce the survival rate of 4T1 cells to 34.3%.The cellular uptake of BTP by 4T1 cells was time-dependent,with the maximum uptake occurring around 12 h.

关 键 词：纳米材料 金属氧化物 声动力治疗 放射治疗 联合治疗 生物工程 

分 类 号：TB34[一般工业技术—材料科学与工程]