电针足三里、太冲穴对功能性消化不良大鼠胃肠动力及NO/sGC/cGMP/PKG1通路的影响  

Effect of electroacupuncture at Zusanli and Taichong points on gastrointestinal motility and NO/sGC/cGMP/PKG1 pathway in rats with functional dyspepsia

作　　者：陈琪 杨德茜 徐派的 潘小丽 CHEN Qi;YANG Deqian;XU Paidi;PAN Xiao Li(College of Acupuncture-Moxibustion and Orthopaedics,Hubei University of Chinese Medicine,Wuhan 430061,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430061

出　　处：《环球中医药》2024年第11期2176-2181,共6页Global Traditional Chinese Medicine

基　　金：湖北省自然科学基金(ZRMS2021001811)。

摘　　要：目的探讨电针对功能性消化不良大鼠胃肠动力及一氧化氮(nitric oxide,NO)/可溶性鸟苷酸环化酶(soluble guanylate cyclase,sGC)/环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)/蛋白激酶G1(protein kinase G1,PKG1)通路的影响。方法将40只SPF级雄性SD大鼠分为正常组、模型组、电针组、抑制剂组和电针+抑制剂组,每组8只。多因素干预法造模后,电针组选择足三里、太冲穴电针30分钟,每天1次,共10天。抑制剂组每天腹腔注射抑制剂ODQ溶液(2 mg/kg),共10天;电针+抑制剂组腹腔注射等量抑制剂后进行电针;正常组与模型组不进行特殊干预。干预结束后检测胃残留率、小肠推进率,苏木素—伊红染色观察胃窦组织形态,蛋白免疫印迹法及实时荧光定量PCR检测胃窦组织sGC、cGMP、PKG1蛋白及mRNA表达水平。结果各组大鼠胃窦组织未见明显器质性改变。与正常组相比,模型组胃残留率明显升高,肠推进率显著降低,胃窦组织sGC、cGMP、PKG1蛋白和mRNA的表达量相较于正常组降低(均P<0.01)。相对于模型组,电针组、抑制剂组和电针+抑制剂组的胃残留率明显降低,小肠推进率明显提高,sGC、cGMP、PKG1蛋白和sGC、cGMP的mRNA表达量升高(均P<0.05)。电针组与电针+抑制剂组胃窦组织sGC、cGMP、PKG1蛋白和sGC、cGMP的mRNA表达量比抑制剂组有所增加(均P<0.05)。结论电针可通过调控NO/sGC/cGMP/PKG1通路改善功能性消化不良大鼠胃肠动力障碍。Objective To investigate the effects of electroacupuncture on gastrointestinal motility and NO/sGC/cGMP/PKG1 pathway in FD rats.Methods The 40 SPF grade male SD rats were divided into the control group,the model group,the EA group,the ODQ group,and the EA+ODQ group,with 8 rats in each group.After modelling by the multifactorial intervention method,the EA group was selected to receive electroacupuncture at the Zusanli and Taichong points for 30 min once a day for 10 days.The ODQ group was injected intraperitoneally with the inhibitor ODQ solution(2 mg/kg)every day for 10 days;the EA+ODQ group was followed after intraperitoneal injection of an equal amount of inhibitor;no special intervention was performed in the control group and the model group.Gastric residual rate and small intestinal propulsion rate were detected after the intervention,HE staining was used to observe the morphology of gastric sinus tissue,and Western blot and RT-qPCR were used to detect the expression levels of sGC,cGMP,and PKG1 proteins and mRNA in gastric sinus tissues.Results No obvious organic changes were observed in the gastric sinus tissue of rats in all groups.Compared with the control group,the gastric residual rate was increased,the intestinal propulsion rate was decreased,and the expression of sGC,cGMP,PKG1 protein and mRNA in the gastric sinus tissue was significantly decreased(all P<0.01).Compared with the model group,the gastric residual rate in the EA,ODQ and EA+ODQ groups was significantly decreased,and the small bowel propulsion rate was significantly increased,and the sGC,cGMP,PKG1 protein and sGC,cGMP mRNA expression were increased(all P<0.05).The sGC,cGMP,PKG1 protein,and sGC,cGMP mRNA expression in gastric sinus tissue were increased in the EA group and the EA+ODQ group compared with the ODQ group(all P<0.05).Conclusion Electroacupuncture improves gastrointestinal dyskinesia in FD rats,and the mechanism may be related to the modulation of NO/sGC/cGMP/PKG1 pathway.

关 键 词：电针 一氧化氮 可溶性鸟苷酸环化酶 环磷酸鸟苷 蛋白激酶G1 功能性消化不良 胃肠运动 

分 类 号：R245[医药卫生—针灸推拿学]