水分胁迫下德兰臭草对内生真菌感染的生理响应  

Physiological responses of Melica transsilvanica to endophytic fungi infection under conditions of water stress

作　　者：张梦梦 王传哲 何嘉坤 施宠[1] ZHANG Mengmeng;WANG Chuanzhe;HE Jiakun;SHI Chong(College of Resource and Environment,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学资源与环境学院,乌鲁木齐830052

出　　处：《生物学杂志》2024年第6期56-61,共6页Journal of Biology

基　　金：国家自然科学基金地区基金项目(31760704)。

摘　　要：为探究不同水分胁迫下德兰臭草对内生真菌感染生理指标的影响,以内生真菌侵染德兰臭草植株(E+)与未侵染植株(E-)为材料进行盆栽控水试验,以土壤含水量60%~65%FWC为对照(CK),并设置土壤含水量85%~90%FWC为高湿处理(W)、15%~20%FWC为干旱处理(D),研究高湿与干旱处理下E+植株与E-植株生理指标的差异。结果表明:在胁迫7 d时,E+植株与E-植株的Pro含量、SOD活性在高湿与干旱条件下均有显著性差异(P<0.05);在胁迫14 d时,E+植株与E-植株的叶绿素含量、Pro含量、MDA含量、SOD活性、POD活性、APX活性和CAT活性在高湿和干旱条件下均有显著性差异(P<0.05);在胁迫21 d时,E+植株的MDA含量、细胞膜相对透性在高湿与干旱条件下均显著高于E-植株(P<0.05)。内生真菌感染可以提高德兰臭草在水分胁迫下的抗性。This study explored the impact of water stress on the physiological responses of Melica transsilvanica to endophytic fungal infection.Using both endophyte-infected(E+)and non-infected(E-)plants,a controlled water experiment with soil moisture levels of 60%-65%field water capacity(FWC)was set up for the control(CK),85%-90%FWC for the high humidity treatment(W),and 15%-20%FWC for the drought treatment(D).The significant differences were found in proline content and superoxide dismutase(SOD)activity between E+and E-plants following 7 days of both high humidity and drought stress(P<0.05).After 14 days of stress,the significant differences were observed in chlorophyll,proline,malondialdehyde(MDA)content,SOD,peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)activities in E+and E-plants under the same conditions(P<0.05).After 21 days of stress,E+plants had significantly increased MDA content and relative cell membrane permeability compared to E-plants under both conditions(P<0.05).The results suggested that endophytic fungal infection enhanced the tolerance of Melica transsilvanica to water stress.

关 键 词：水分胁迫 内生真菌 德兰臭草 生理指标 保护酶 

分 类 号：Q945.17[生物学—植物学] Q949.32Q945.78