1株鸡传染性喉气管炎病毒的分离鉴定及致病性评价  

Isolation,identification and phylogenetic analysis of avian infectious laryngotracheitis virus

作　　者：张亚 胡小飞 田辉 段佳蕾 田克恭 张云静 张海洋 逄文强 Zhang Ya;Hu Xiaofei;Tian Hui;Duan Jialei;Tian Kegong;Zhang Yunjing;Zhang Haiyang;Pang Wenqiang(National Reasearch Center For Veterinary Medicine,Henan Luoyang 471000;Pulike Biological Engineering INC,Henan Luoyang 471000)

机构地区：[1]国家兽用药品工程技术研究中心,河南洛阳471000 [2]普莱柯生物工程股份有限公司,河南洛阳471000

出　　处：《现代畜牧兽医》2024年第11期1-5,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：“河洛英才计划”河洛创新人才项目:家禽新型基因工程多联多价疫苗的研发与产业化;2023年洛阳市重大科技专项:畜禽重要疫病新型疫苗研发与产业化(2301014A)。

摘　　要：研究旨在为当前国内鸡传染性喉气管炎病毒(infectious laryngotracheitis virus,ILTV)的流行情况调查及相关疫苗研发提供参考。河南省濮阳市某养鸡场的蛋鸡疑似感染ILTV,将阳性病料处理后接种鸡胚绒毛尿囊膜,盲传3代后产物进行PCR鉴定和TK、gD基因序列测定分析,并通过动物回归试验初步研究分离株的致病性。结果显示,盲传3代后绒毛尿囊膜出现痘斑、增厚现象,产物PCR鉴定结果为阳性,将其命名为PY株。PY株TK、gD核苷酸序列与GenBank发表的ILTV序列相似性分别为99.4%~100.0%、99.1%~100.0%。49日龄SPF鸡于攻毒后第3 d出现鸡传染性喉气管炎的典型临床症状,剖检病死鸡可见喉头气管内充满血样黏液。攻毒后鸡发病率为100%、死亡率为30%。研究表明,分离株PY株是一株致病力较强的ILTV。The purpose of this study was to provide a reference for the current epidemic investigation of infectious laryngotracheitis virus(ILTV)in chickens in China and related vaccine development.Laying hens suspected to be infected with ILTV in a chicken farm in Puyang City,Henan Province were treated with positive samples and inoculated with chicken embryo chorioallasic membrane.After blind transmission for three generations,the products were identified by PCR and analyzed by TK and gD gene sequences.The pathogenicity of the isolates was preliminarily studied by animal regression test.The results showed that the chorioallantoic membrane had acne spots and thickening after three generations of blind transmission,and the product was identified positive by PCR and named PY strain.The similarity of TKand gD nucleotide sequences of PY strain to ILTV sequences published by GenBank was 99.4%~100.0%and 99.1%~100.0%,respectively.The typical clinical symptoms of infectious laryngotracheitis were found in 49-day-old SPF chickens on the 3rd day after the challenge.The morbidity and mortality of chickens were 100%and 30%respectively.The study indicates that the isolated PY strain is an ILTV with strong pathogenicity.

关 键 词：鸡传染性喉气管炎病毒 TK基因 GD基因 致病性 

分 类 号：S852.65[农业科学—基础兽医学]