HPLC法测定麻杏石甘口服液中甘草酸含量  

Determination of glycyrrhizic acid in Maxing Shigan oral liquid by HPLC

作　　者：张趁 杜西翠 李会会 刘格格 耿天姿 耿璐 郑美如 徐丹 Zhang Chen;Du Xicui;Li Huihui;Liu Gege;Geng Tianzi;Geng Lu;Zheng Meiru;Xu Dan(Hebei Jinkun Animal Pharmaceutical Co.Ltd.,Hebei Xinji 052360;Shijiazhang Huifeng Animal Health Products Co.Ltd.,Hebei Xinji 052360;Hebei Veterinary Medicine Preparation Technology Innovation Center,Hebei Xinji 052360;Wucheng County Animal Husbandry and Fishery Development Center,Shangdong Dezhou 253300)

机构地区：[1]河北锦坤动物药业有限公司,河北辛集052360 [2]石家庄市汇丰动物保健品有限公司,河北辛集052360 [3]河北省兽用中药制剂技术创新中心,河北辛集052360 [4]武城县畜牧渔业发展中心,山东德州253300

出　　处：《现代畜牧兽医》2024年第11期21-24,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：试验旨在建立1种高效液相色谱法(HPLC)测定麻杏石甘口服液中甘草酸含量的分析方法。样品经流动相提取、过滤,注入高效液相色谱仪进行检测。色谱柱为Agilent ZORBAX SB-C18 (4.6 mm×250 mm,5μm),以甲醇-0.2 mol/L乙酸铵溶液-冰乙酸(体积比为67∶33∶1)为流动相,检测波长为250 nm,流速1.0 mL/min,柱温30℃,进样量10μL。结果表明,该方法在0.1~1.0 g/L范围内线性良好,甘草酸含量的线性方程为Y=7 767.13X-63.247,r=0.999 8。该方法具有较好的精密度、准确度和重复性,加样回收率为92.86%~96.19%,相对标准偏差值(RSD)均小于2.0%,适用于测定麻杏石甘口服液中甘草酸含量。The aim of this study was to establish a method for the determination of glycyrrhizic acid in Maxing Shigan oral liquid by high performance liquid chromatography(HPLC).The samples were extracted by mobile phase,filtered and injected into high performance liquid chromatography for detection.The determination was performed on Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)column with methanol-0.2 mol/L ammonium acetate solution-glacial acetic acid(the volume ratio was 67∶33∶1)as the mobile phase.The detection wavelength was set at 250 nm,the flow rate was 1.0 mL/min,and the column temperature was 30℃,the sample size was 10μL.The results show that the method has good linearity in the range of 0.1~1.0 g/L,and the linear equation of glycyrrhizic acid was Y=7767.13X-63.247,r=0.9998.The method has good precision,accuracy and repeatability.The recoveries were 92.86%~96.19%,and the relative standard deviations(RSD)are less than 2.0%.It is suitable for the determination of glycyrrhizic acid in Maxing Shigan oral liquid.

关 键 词：HPLC法 麻杏石甘口服液 甘草酸 

分 类 号：S853.7[农业科学—临床兽医学]