不同温度处理下内生真菌对宿主布顿大麦生长和生理指标的影响  

Effects of Endophytic Fungi on Growth and Development of Host Hordeum bogdanii under Different Temperatures

作　　者：陈胜 龙凤 户萌菲 韩丹 王凯 陈水红[1] 韩秀锋[1] Chen Sheng;Long Feng;Hu Mengfei;Han Dan;Wang Kai;Chen Shuihong;Han Xiufeng(College of Life Sciences and Technology,Tarim University/Xinjiang Production&Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Alar 843300,China;Shenzhen Advanced Technology Research Institute,Chinese Academy of Sciences,Shenzhen 518055,China;Tarim Vocational and Technical College,Alar 843300,China)

机构地区：[1]塔里木大学生命科学与技术学院/塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300 [2]中国科学院深圳先进技术研究院,广东深圳518055 [3]塔里木职业技术学院,新疆阿拉尔843300

出　　处：《山东农业科学》2024年第11期61-67,共7页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31660689,32260356);塔里木大学重点培育项目(TDZKZD202201);塔里木大学研究生科研创新项目(TDGRI202207)。

摘　　要：为了探明不同温度处理下内生真菌对布顿大麦(Hordeum bogdanii)生长和理化特性的影响,本试验以新疆温宿县布顿大麦为对象,研究10℃、15℃和20℃处理3、7 d和14 d带内生真菌(E+)和不带内生真菌(E-)布顿大麦株高、分蘖数、丙二醛(MDA)含量、脯氨酸含量以及过氧化氢酶(CAT)、过氧化物酶(POD)活性的变化情况。结果显示:10℃和15℃处理下,E+植株株高和分蘖数显著高于E-植株;处理3 d和7 d,随着温度降低,E+植株的POD活性不断增加,并且10℃时均显著高于E-植株;3个温度处理下E+植株的CAT活性均高于E-植株,且均在15℃时达到显著水平;10℃和15℃处理下,E+植株的丙二醛含量低于E-植株,脯氨酸含量高于E-植株,且分别在15、10℃达显著水平。综上表明,布顿大麦内生真菌可通过提高抗氧化酶活性、促进渗透调节物质积累和降低膜损伤来增强宿主对不同温度环境的适应性。In order to investigate the effects of endophytic fungi on growth and physiological characteris-tics of Hordeum bogdanii under different temperatures,this experiment was carried out focusing on H.bogdanii from Wensu County,Xinjiang.The changes of plant height,tiller number,malondialdehyde(MDA)content,proline content,and activities of catalase(CAT)and peroxidase(POD)in H.bogdanii plants with endophyt-ic fungi(E+)and without endophytic fungi(E-)were studied after 3,7 and 14 days of 10,15℃and 20℃treatments.The results showed that the plant height and tiller number of E+plants were significantly higher than those of E-plants at 10℃and 15℃.After 3 and 7 days of different treatments,the POD activity of E+plants continuously increased with temperature decreasing and was significantly higher than that of E-plants at 10℃.The CAT activity of E+plants was higher than that of E-plants under the three temperature treat-ments,and reached significant level at 15℃.Under 10℃and 15℃treatments,the MDA content of E+plants was lower than that of E-plants,while the proline content was higher than that of E-plants,which reached significant level at 15℃and 10℃,respectively.In summary,the endophytic fungi of H.bogdanii could enhance the adaptability of host to different temperature environment by increasing the activities of antioxi-dant enzymes,promoting the accumulation of osmotic adjustment substances and reducing membrane damage.

关 键 词：布顿大麦 内生真菌 温度 抗氧化酶活性 

分 类 号：S543.901[农业科学—作物学]