淡紫拟青霉和球孢白僵菌对番茄烟粉虱若虫的毒力评价  

Virulence Evaluation of Paecilomyces lilacinus and Beauveria bassiana Against Tomato Bemisia tabaci Nymphs

作　　者：郝轩卉 刘守柱[2] HAO Xuanhui;LIU Shouzhu(College of Plant Protection,Shandong Agricultural University,Taian 271018,China;College of Agriculture and Biology,Liaocheng University,Liaocheng 252059,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]聊城大学农业与生物学院,山东聊城252059

出　　处：《蔬菜》2024年第12期46-51,共6页Vegetables

基　　金：山东省自然科学基金项目(ZR2020MC127)。

摘　　要：为探究淡紫拟青霉和球孢白僵菌对烟粉虱若虫的防治效果,选用市面上常见的淡紫拟青霉和球孢白僵菌粉剂,以噻虫嗪作为对照药剂,采用浸渍法测定了2种生防菌对番茄叶片上烟粉虱若虫的毒力。结果表明:淡紫拟青霉各浓度均不能有效抑制烟粉虱若虫的羽化,若虫羽化率高,显示对烟粉虱若虫无致病力;药后1~3 d球孢白僵菌各浓度亦不能有效抑制烟粉虱若虫羽化,但4~5 d后用1×10^(9)个孢子/mL处理的烟粉虱若虫羽化率明显降低,若虫死亡且虫体体表生长出明显的白色菌丝,显示出明显的致病力;药后5 d球孢白僵菌对烟粉虱若虫表现出明显的致死效果,防治效果为58.85%,LC_(50)为7.72×10^(8)个孢子/mL。经方差分析,球孢白僵菌与对照药剂噻虫嗪药效相当。综上,淡紫拟青霉不能用于烟粉虱若虫的防治,球孢白僵菌可以用于烟粉虱若虫的防治,但需要较高的剂量,适宜的浓度为≥1×10^(9)个孢子/mL。To investigate the prevention and control effect of Paecilomyces lilacinus and Beauveria bassiana against Bemisia tabaci nymphs,commonly available powder formulations of these biocontrol agents were selected,with thiamethoxam as control.The virulence of the two biocontrol agents against B.tabaci nymphs on tomato leaves was evaluated using the dipping method.The results showed that P.lilacinus at all tested concentrations did not effectively inhibit the emergence of the nymphs,resulting in a high emergence rate and indicating no pathogenicity against the nymphs.Similarly,B.bassiana at all tested concentrations did not effectively inhibit nymphs emergence from 1 to 3 days post-treatment.However,from 4 to 5 days post-treatment,the emergence rate of nymphs treated with 1×10^(9) spores/mL of B.bassiana significantly decreased,with visible white mycelium growing on nymph's bodies,indicating a distinct pathogenicity.By day 5,B.bassiana showed a significant lethal effect on the nymphs,with the control effect of 58.85% and LC_(50) of 7.72×10^(8) spores/mL.Variance analysis indicated that the control effect of B.bassiana was similarly to that of control with thiamethoxam treatment.In conclusion,P.lilacinus is not suitable for controlling B.tabaci nymphs,while B.bassiana can be used for their control,requiring a relatively high dose,with an optimal concentration≥1×10^(9) spores/mL.

关 键 词：淡紫拟青霉 球孢白僵菌 烟粉虱 若虫 毒力 防治效果 番茄 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]