黔产野生槲蕨内生菌群多样性及功能挖掘  

Diversity and Functional Exploration of Endophytic Microbiota in Wild Drynaria roosii from Guizhou

作　　者：张翔宇 陈晓芳 柳敏 周茂嫦 王永 陈睿 洪林 梁小伟 Zhang Xiangyu;Chen Xiaofang;Liu Min;Zhou Maochang;Wang Yong;Chen Rui;Hong Lin;Liang Xiaowei(Bijie Institute of Traditional Chinese Medicine,Bijie 551700,China;Bijie Medical College,Bijie 551700,China;Bijie Agricultural Investment Endangered Species Breeding Technology Co.,Ltd.,Bijie 551700,China;Bijie National Reserve Forest Management Co.,Ltd.,Bijie 551700,China)

机构地区：[1]毕节市中药研究所,贵州毕节551700 [2]毕节医学高等专科学校,贵州毕节551700 [3]毕节市农投濒危物种繁育科技有限公司,贵州毕节551700 [4]毕节市国储林管理有限公司,贵州毕节551700

出　　处：《中国野生植物资源》2024年第11期72-80,共9页Chinese Wild Plant Resources

基　　金：贵州省科技计划项目(黔科合支撑[2023]一般003);广州市科技计划项目(2023B03J1292);贵州省人才基地项目(RCJD2020-21);贵州省高层次创新型人才项目(毕科人才合字[2021]05号);企业横向项目(GCLGS2022-04);毕节市优势中药材创新人才团队项目(毕委人领办通[2023]16号);毕节市科技创新平台及人才团队(毕科合[2023]66号)。

摘　　要：目的:揭示黔产槲蕨内生菌多样性,为利用有益菌群提高栽培效益提供依据。方法:以黔产野生槲蕨块茎为材料,采用高通量测序和生物信息学分析内生菌群落多样性、网络关系、功能预测分析。结果:6个区域槲蕨样本共聚类到真菌39个纲下的646属,细菌104个纲下的1007属;真菌共有OTU为49个,细菌共有OTU为1093个;主要优势真菌为座囊菌纲、粪壳菌纲、散囊菌纲等,优势细菌为放线菌纲、变形菌纲等;网络分析揭示大部分内生菌属之间存在较高的正相关性;槲蕨内生真菌中腐生营养型菌属有24属,共生营养型有20属,病理营养型有9属,病理-腐生营养型有4属,病理-共生营养型仅1属;内生细菌中各表型最高占比分别为需氧型KL(86.34%)、厌氧型CS(7.73%)、移动元件ZA(55.96%)、兼性厌氧WD(22.12%)、生物膜形成NY(22.36%)、革兰氏阴性NY(55.41%)、革兰氏阳性KL(75.75%)、潜在致病性ZA(70.16%)、胁迫耐受WD(29.69%);菌群中的Dactylonectria通常为根腐病致病菌,贪噬菌属可能通过与根际环境互作维持槲蕨根系正常发育,鞘氨醇单胞菌可能通过固氮、溶解磷酸盐、产生植物生长激素等从而提供槲蕨生长所需营养物质促进正常生长。结论:槲蕨有益内生菌群对于维持其正常生长发育具有重要意义,可研究利用这些有益菌群提高槲蕨栽培成活率。Objective:This study aimed to reveal the diversity of endophytic microbiota in Drynaria roosii from Guizhou,and provide a basis for utilizing beneficial microbial communities to improve cultivation efficiency.Methods:Wild D.roosii tubers from Guizhou were used as materials.High-throughput sequencing and bioinformatics analysis were employed to analyze the diversity,network relationships,and functional prediction of endophytic microbial communities.Results:The D.roosii samples from 6 regions were classified into 39 classes and 646 genera of fungi,and 104 classes and 1007 genera of bacteria.The operational taxonomic units(OTUs)of fungi and bacteria were 49 and 1093,respectively.The main dominant fungi were Dothideomycetes、Sordariomycetes、Eurotiomycetes,while the dominant bacteria were Actinobacteria and Proteobacteria.Network analysis revealed a high positive correlation between most endophytic microbial genera.Among the D.roosii endophytic fungi,there were 24 genera of saprophytic fungi,20 genera of symbiotic fungi,9 genera of pathogenic fungi,4 genera of pathogenic-saprophytic fungi,and only 1 genus of pathogenic-symbiotic fungi.The highest proportions of various phenotypes in endophytic bacteria were aerobic KL(86.34%),anaerobic CS(7.73%),mobile element ZA(55.96%),facultative anaerobic WD(22.12%),biofilm-forming NY(22.36%),Gram-negative NY(55.41%),Gram-positive KL(75.75%),potentially pathogenic ZA(70.16%),and stress-tolerant WD(29.69%).Dactylonectria in the microbiota was commonly a pathogenic fungi for root rot.Variovorax may maintain the normal development of D.roosii roots by interacting with the rhizosphere environment.Sphingomonas may provide the necessary nutrients for the growth of D.roosii through nitrogen fixation,phosphate solubilization.And production of plant growth hormones to promote normal growth.Conclusion:The beneficial endophytic microbiota was of great significance for maintaining normal growth and development of D.roosii.And further research can be conducted to utilize these beneficial mi

关 键 词：槲蕨 内生菌 多样性 功能 

分 类 号：R282.2[医药卫生—中药学]