Morphological characteristics,developmental dynamics,and gene temporal expressions across various development stages of Aphis gossypii sexual female  

作　　者：LÜJingli WANG Liuyu ZHANG Kaixin LI Dongyang GAO Mengxue GUO Lixiang TANG Zhijuan GAO Xueke ZHU Xiangzhen WANG Li JI Jichao LUO Junyu CUI Jinjie 

机构地区：[1]Bio-Breeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China [2]State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization,School of Agri Research Base of Zhengzhou University,State Key Laboratory of Cotton cultural Sciences,Zhengzhou University,Zhengzhou,Henan 450001,China [3]Western Agricultural Research Center,Chinese Academy of Agricultural Sciences,Changji,Xinjiang 831100,China [4]Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China [5]State Key Laboratory of Plant Stress Biology,College of Life Sciences,Henan University,Kaifeng,Henan 450046,China [6]College of Horticulture and Landscape Architecture,Tianjin Agricultural University,Tianjin 300392,China.

出　　处：《Journal of Cotton Research》2024年第4期372-385,共14页棉花研究（英文）

基　　金：funded by National Natural Science Foundation of China(No.32102214);Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences;China Agriculture Research System(CARS-15-21);National Key R&D Program of China(2022YFD1400300)。

摘　　要：Background Aphis gossypii(Hemiptera:Aphididae)is a worldwide polyphagous phloem-feeding agricultural pest,and it can produce offspring by sexual or asexual reproduction.Compared with dozens of generations by parthenogenesis,sexual reproduction is performed in only one generation within one year,and little is known about the sexual reproduction of A.gossypii.In this study,sexual females of A.gossypii were successfully obtained through a previously established induction platform,and the morphological characteristics,developmental dynamics,and temporal gene expression were examined.Subsequently,signaling pathways potentially involved in regulating the growth,development,and reproduction of sexual females were investigated.Results The morphological observation showed that from the 1st instar nymph to adult,sexual females exhibited a gradually deepened body color,an enlarged body size,longer antennae with a blackened end,and obviously protruding cauda(in adulthood).The anatomy found that the ovaries of sexual females developed rapidly from the 2^(nd)instar nymph,and the embedded oocytes matured in adulthood.In addition,time-course transcriptome analysis revealed that gene expression profiles across the development of sexual females fell into 9 clusters with distinct patterns,in which gene expression levels in clusters 1,5,and 8 peaked at the 2^(nd)instar nymphal stage with the largest number of up-regulated genes,suggesting that the 2^(nd)instar nymph was an important ovary development period.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that a large number of genes in the sexual female adult were enriched in the TGF-beta signaling pathway and Forkhead box O(FoxO)signaling pathway,highlighting their important role in sexual female adult development and reproduction.Conclusion The morphological changes of the sexual female at each developmental stage were revealed for the first time.In addition,time-course transcriptomic analyses suggest genes enriched in the TGF-beta signaling pathw

关 键 词：Aphis gossypii Sexual female Growth and development OOGENESIS Time-course transcriptome 

分 类 号：S562[农业科学—作物学]