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出 处:《Journal of Cotton Research》2024年第4期414-424,共11页棉花研究(英文)
摘 要:Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use efficiency of cotton to induce K deficiency.Results:The cotton variety,Jihe 321(wild type,WT)and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317,OE 29312)were studied in field with low soil-test K~+(47.8 mg·kg~(-1)).In the field with low soil-test K~+,only OE 29317 had less biomass and K~+accumulation than the WT at some growth stages.Both Bt lines produced similar or even greater seed cotton yield than WT in the field.When the Bt gene(~70%)in OE-29317 and OE-29312 plants was silenced by virus-induced gene silencing(VIGS),the VIGS Bt plants did not produce more biomass than VIGSgreen fluorescent protein(control)plants.Conclusions:The introduction of Bt gene did not necessarily hinder the K use efficiency of the cotton lines under this study.Keywords:Biomass,Bt(Bacillus thuringiensis)gene,Cotton(Gossypium hirsutum L.),K uptake,K utilization index,Yield。
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