Tetraphenylethylene-doped covalent organic frameworks as a highly efficient aggregation-induced electrochemiluminescence emitter for ultrasensitive miRNA-21 analysis  

作　　者：Wen-Yi Shu Ruiqi Su Linli Yao Yao Xiao Miao-Miao Chen Wei Wen Shengfu Wang Chengyi Xiong Xiuhua Zhang 

机构地区：[1]Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry,Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China [2]State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan 430062,China

出　　处：《Advanced Sensor and Energy Materials》2023年第2期19-26,共8页先进传感器与能源材料（英文）

基　　金：National Natural Science Foundation of China(Nos.42275152,32072305,22076042);Special Project for Hubei Local Science and Technology Development Guided by the Central Government(No.2019ZYYD004);Open Project Funding of the State Key Laboratory of Biocatalysis and Enzyme Engineering,Knowledge Innovation Program of Wuhan-Shuguang Project(2022020801020331);Foundation for Creative Research Groups of Hubei Province of China(No.2011CDA111).

摘　　要：MicroRNAs(miRNAs)as a well-known kind of cancer marker are closely associated with the formation and metastasis of tumors.Here,a novel tetraphenylethylene(TPE)-doped covalent organic frameworks(TPE-COFs)with strong aggregation-induced electrochemiluminescence(AIECL)response was synthesized and introduced to construct an ultrasensitive biosensor for the detection of miRNA-21.The strong aggregation-induced emission(AIE)response was obtained because the molecular motion of TPE was restricted by COFs which had the porosity and highly ordered topological structure.Meanwhile,the porous structure of COFs allowed TPE to react with electrochemiluminescence(ECL)coreactants more effectively.Furthermore,COFs significantly improved the electron transport efficiency of the entire ECL system.All of these endowed the TPE-COFs with superior AIECL performance.Then,a TPE-COFs based ECL resonance energy transfer(ECL-RET)system was constructed for ultrasensitive miRNA-21 biosensing with differential signal readout.The proposed assays exhibited excellent sensitivity with a wide dynamic range from 10 aM to 1 pM and a low detection limit of 2.18 aM.Therefore,these indicated that doping TPE in COFs was a creative way to develop functional COFs and provided an effective way for enhancing AIECL.Furthermore,this work boarded the application of AIECL in analytical chemistry.

关 键 词：Aggregation-induced ELECTROCHEMILUMINESCENCE Covalent organic frameworks Resonance energy transfer MICRORNA-21 Biosensor 

分 类 号：TQ3[化学工程]