Kindlin-3和β2整合素在急性冠脉综合征患者中的表达水平及作用机制  

Expression level and mechanism of Kindlin-3 andβ2 integrin in patients with acute coronary syndrome

作　　者：鲁兆娜 周青 李文 吴校林[2] LU Zhaona;ZHOU Qing;LI Wen;WU Xiaolin(College of Medicine,Wuhan University of Science and Technology,Hubei Province,Wuhan430000,China;Department of Cardiovascular,Xiangyang Central Hospital Affiliated to Hubei University of Arts and SciencesInstitute of Cardiovascular Disease,Hubei University of Arts and Sciences,Hubei Province,Xiangyang441000,China)

机构地区：[1]武汉科技大学医学部医学院,湖北武汉430000 [2]湖北文理学院附属襄阳市中心医院心血管内科湖北文理学院心血管病研究所,湖北襄阳441000

出　　处：《中国医药导报》2024年第25期1-7,共7页China Medical Herald

基　　金：湖北省卫生健康委员会青年人才项目(WJ2021Q013)。

摘　　要：目的探讨急性冠脉综合征(ACS)患者外周血中Kindlin-3和β_(2)整合素的表达水平及相关作用机制。方法选取2023年9月至2024年1月湖北文理学院附属襄阳市中心医院收治的ACS患者80例,其中不稳定型心绞痛(UA)患者30例(UA组)、急性ST段抬高型心肌梗死(STEMI)患者26例、急性非ST段抬高型心肌梗死(NSTEMI)患者24例,后两者合称为急性心肌梗死(AMI)组。冠状动脉造影正常患者20例(正常组)和稳定型心绞痛(SAP)患者30例(SAP组)均作为对照。通过流式细胞术检测外周血白细胞中β_(2)整合素(LFA-1和Mac-1)的表达,采用酶联免疫吸附试验法检测静脉血清中Fractalkine、ICAM-1、C反应蛋白(CRP)及白细胞介素-6(IL-6)水平,采用Western blot法检测外周血白细胞中Kindlin-3、FAK、AKT蛋白表达水平。结果AMI组患者外周血白细胞计数(WBC)、中性粒细胞计数(NEUT)水平高于其他三组(P<0.05);UA组和AMI组患者外周血白细胞中LFA-1和Mac-1阳性细胞百分率高于正常组和SAP组(P<0.05);UA组血清Fractalkine、ICAM-1、IL-6水平高于正常组和SAP组(P<0.05);AMI组血清Fractalkine、ICAM-1、CRP、IL-6水平高于其他三组(P<0.05)。FAK蛋白在UA组、AMI组中的水平高于正常组和SAP组(P<0.05);AKT蛋白在UA组、AMI组中的水平均高于正常组和SAP组(P<0.05)。SAP组的FAK和AKT蛋白水平高于正常组(P<0.05)。Kindlin-3蛋白在SAP组和UA组中的表达水平高于正常组(P<0.05),但在AMI组却低于正常组(P<0.05)。结论ACS时Kindlin-3和β_(2)整合素表达增加,伴随信号通路FAK-PI3K/AKT激活及炎症因子释放,提示其参与ACS的炎症反应。Objective To explore Kindlin-3 andβ_(2) integrin expression levels in the peripheral blood of patients with acute coronary syndrome(ACS)and the associated mechanisms.Methods A total of 80 patients with ACS admitted to Xiangyang Central Hospital Affiliated to Hubei University of Arts and Sciences from September 2023 to January 2024 were selected,including 30 patients with unstable angina pectoris(UA)as UA group,26 patients with acute ST segment elevation myocardial infarction(STEMI),and 24 patients with acute non-ST segment elevation myocardial infarction(NSTEMI),the latter two were collectively known as the acute myocardial infarction(AMI)group.Additionally,twenty patients had normal coronary angiography and thirty patients had stable angina pectoris(SAP),forming the control.Flow cytometry was used to detect the expression ofβ_(2) integrins(LFA-1 and Mac-1)in peripheral blood leukocytes.Enzyme linked immunsorbent assay was used to measure the levels of fractalkine,ICAM-1,C-reactive protein(CRP),and interleukin-6(IL-6)in venous serum Western blot was used to determine the levels of Kindlin-3,FAK,and AKT proteins in peripheral blood leukocytes.Results Patients in the AMI group had higher levels of peripheral blood white blood cell count(WBC)and neutrophils count(NEUT)than those in the other three groups(P<0.05).The percentage of LFA-1 and Mac-1 positive cells in peripheral blood leukocytes of patients in the UA and AMI groups was higher than that of patients in the normal and SAP groups(P<0.05);the serum levels of Fractalkine,ICAM-1,and IL-6 were higher in patients in the UA group than those in the normal and SAP groups(P<0.05);the serum levels of Fractalkine,ICAM-1,CRP,and IL-6 were higher in patients in the AMI group than the other three groups(P<0.05).The levels of FAK proteins were higher in the UA group and AMI group than the normal and SAP groups(P<0.05);AKT protein levels were higher in the UA and AMI groups than the normal and SAP groups(P<0.05).The levels of FAK and AKT proteins were higher in the SAP gr

关 键 词：急性冠脉综合征 Kindlin-3 Β2整合素 炎症 

分 类 号：R541.4[医药卫生—心血管疾病]