Coating tetrahedral DNA framework with endosomolytic peptides for improved stability and cytosolic delivery  

作　　者：Jinjun He Xiang Ji Zihui Xu Wei He Yan Zhao Lele Sun Lan Ma 

机构地区：[1]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518132,China [2]Institute of Materiobiology,Department of Chemistry,College of Science,Shanghai University,Shanghai 200444,China [3]Tsinghua-Berkeley Shenzhen Institute,Tsinghua University,Shenzhen,518055 China [4]Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [5]Division of Physical Biology Department,CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China [6]State Key Laboratory of Chemical Oncogenomics,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China

出　　处：《Advanced Sensor and Energy Materials》2024年第2期1-5,共5页先进传感器与能源材料（英文）

基　　金：supported by the National Key R&D Program of China(2020YFA0908900);Universities Stable Funding Key Projects(WDZC20200821104802001);the State Key Laboratory of Chemical Oncogenomics;the Institute of Biomedical Health Technology and Engineering of Shenzhen Bay Laboratory;GuangDong Basic and Applied Basic Research Foundation(2021A1515110819).

摘　　要：DNA nanostructures have emerged as promising carriers for drug delivery.However,challenges such as low stability,poor cellular uptake efficiency,and vulnerability to lysosomal degradation still hinder their therapeutic potential.In this study,we demonstrate the coating of tetrahedral DNA frameworks(TDF)with the endosomolytic peptide L17E through electrostatic interactions to address these issues.Our findings highlight that L17E coating substantially enhances the stability of TDFs and improves their uptake efficiency into RAW264.7 cells through endocytosis and macropinocytosis.Moreover,L17E coating enables efficient endosomal release of TDFs.Finally,we employed L17E-coated TDF to deliver osteogenic growth peptide and demonstrated its potential applications in inhibiting periodontitis both in vitro and in vivo.This straightforward and cost-effective strategy holds promise for advancing the biomedical applications of DNA nanostructures.

关 键 词：Tetrahedral DNA framework Endosomolytic peptides STABILITY Endosomal escape Efficient delivery 

分 类 号：TG1[金属学及工艺—金属学]