离体肝脏长期灌注系统的构建  

Establishment of a long-term Ex situ liver perfusion system

作　　者：刘之颖 陈宏珲 李冶夫 陈志涛 杨永琪 赵强[1] 何晓顺[1] Liu Zhiying;Chen Honghui;Li Yefu;Chen Zhitao;Yang Yongqi;Zhao Qiang;He Xiaoshun(Organ Transplant Center,The First Affiliated Hospital of Sun Yat-sen University,Guangdong Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院器官移植中心,广东广州510080

出　　处：《实用器官移植电子杂志》2024年第6期492-497,共6页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：国家自然科学基金(82270686)。

摘　　要：目的使用常温机械灌注(normothermic machine perfusion,NMP)建立离体肝脏长期灌注系统(ex situ MP for long-term liver perfusion)。方法将1例子猪全肝连接至Life-X100灌注系统进行常温机械灌注,持续监测灌注过程中灌注参数及灌注液血气分析结果,评估离体肝脏的功能。结果成功离体灌注猪肝138 h,各项灌注参数稳定,离体肝脏功能维持良好。结论我们初步建立了离体肝脏长期灌注系统,在未来可用于人类肝脏的离体养护,并在此基础上进行多项探索性研究,促进我国医学发展。Objective To establish an ex situ machine perfusion(MP)system for long-term liver perfusion using normothermic machine perfusion(NMP)technology.Methods A porcine liver was connected to the Life-X100 perfusion system for normothermic machine perfusion.Perfusion parameters and perfusate blood-gas analysis results were continuously monitored during the perfusion process to assess the ex situ liver function.Results The porcine liver was successfully perfused ex situ for 138 hours,with stable perfusion parameters and well-maintained liver function.Conclusion We have preliminarily established an ex situ long-term liver perfusion system.This system can be used for the ex situ maintenance of human livers in the future and can serve as a basis for various exploratory studies to advance medical development in our country.

关 键 词：长期常温机械灌注 离体肝脏保存 器官功能维护 器官医学 

分 类 号：R657.3[医药卫生—外科学]