枣DCL基因家族注释及其在枣疯病发生中的表达模式分析  

Annotation of jujube DCL gene family and its expression pattern during jujube witches’broom disease

作　　者：张亚婕 黄玉静 姚佳 曹懂懂 李君毅 王梦丽 李萌 李继东[1,3] 冯建灿 ZHANG Yajie;HUANG Yujing;YAO Jia;CAO Dongdong;LI Junyi;WANG Mengli;LI Meng;LI Jidong;FENG Jiancan(College of Forestry,Henan Agricultural University,Zhengzhou 450046,China;The Scientific and Technological Information Center of Henan,Zhengzhou 450003,China;Shelter Forest Engineering Research Center of the National Forestry and Grassland Administration,Zhengzhou 450046,China;College of Horticulture,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学林学院,河南郑州450046 [2]河南省科学技术情报中心,河南郑州450003 [3]国家林业和草原局农田防护林工程技术研究中心,河南郑州450046 [4]河南农业大学园艺学院,河南郑州450046

出　　处：《河南农业大学学报》2024年第6期936-945,共10页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(32071803);河南省自然科学基金项目(232300420007);河南省科技兴林项目(2023008)。

摘　　要：【目的】从枣基因组中注释枣DCL(dicer-like)基因家族成员,对其在枣疯病发生过程中的表达模式进行分析,研究枣DCL基因家族在响应植原体侵染中的作用。【方法】从NCBI网站下载冬枣基因组(Genebank号JREP00000000)数据,以拟南芥DCL蛋白序列为探针,使用生物信息学方法筛选得到枣DCL家族成员,分析其染色体位置、基因及蛋白结构、系统进化和保守基序特征,对枣DCL基因家族成员在枣疯病发生过程中的转录组数据进行分析,并对各成员在枣疯病发生过程中的动态进行实时定量反转录聚合酶链反应(qRT-PCR)定量。【结果】以4个拟南芥DCL蛋白为探针,注释了11个枣DCL成员(ZjDCLs),其蛋白分子质量范围为15 217.48~225 623.49 D,等电点为5.82~9.05。11个枣DCL基因不均匀分布在6条枣染色体上,其中1号染色体上的最多。根据和拟南芥4个DCL的进化关系可将11个枣DCL成员分为4类。基因结构分析表明,ZjDCL3和ZjDCL7外显子数量最多为25个,保守基序分析表明,枣DCL的结构较为保守。转录组数据分析表明,10个枣DCL基因在枣疯病发生过程中有表达,ZjDCL6在第Ⅳ个时期的表达量最高,qRT-PCR定量分析表明,ZjDCL6,ZjDCL8和ZjDCL10在不同时期健康树和病树之间差异变化较大。【结论】枣基因组中有11个DCL基因家族成员,结构较为保守,其中ZjDCL6,ZjDCL8和ZjDCL10在枣响应植原体侵染中起重要作用。【Objective】The members of the jujube DCL gene family were annotated from the jujube genome,and their expression patterns in the occurrence of jujube witches’broom disease were analyzed,and the role of the jujube DCL gene family in responding to phytoplasma infection was studied.【Method】The genome data of Jujube(Genebank No.JREP00000000)was downloaded from the NCBI website,and the DCL family members of Jujube were screened by bioinformatics methods using the DCL sequence of Arabidopsis thaliana as probe,and their chromosomal position,gene and protein structure,phylogenetic and conserved motif characteristics were analyzed,the transcriptome data of the DCL gene family members of jujube during the progress of jujube witches’broom disease were analyzed,and the dynamics of each member in the process of jujube witches’broom disease were quantified by qRT-PCR.【Result】Eleven jujube DCLs(ZjDCLs)were annotated using four Arabidopsis DCL proteins as probes,with molecular weights ranging from 15217.48~225623.49 Da and isoelectric points ranging from 5.82~9.05.The 11 jujube DCL genes were unevenly distributed on 6 jujube chromosomes,most of which were on chromosome 1,and the 11 jujube DCLs could be divided into 4 categories according to the evolutionary relationship with the 4 DCLs in Arabidopsis,and the gene structure analysis showed that the number of ZjDCL3 and ZjDCL7 exons was up to 25,and the conserved motif analysis showed that the structure of jujube DCL was relatively conserved.Transcriptome data analysis showed that 10 jujube DCL genes were expressed during the process of jujube witches’broom disease,and the expre-ssion level of ZjDCL6 was the highest in theⅣperiod,and qRT-PCR quantitative analysis showed that ZjDCL6,ZjDCL8 and ZjDCL10 were significantly different between healthy and diseased trees at diffe-rent periods.【Conclusion】There were 11 DCL gene family members in the jujube genome,and the structure was relatively conserved,among which ZjDCL6,ZjDCL8 and ZjDCL10 played an important ro

关 键 词：枣疯病 植原体 DCL基因家族 植物防御 表达模式 

分 类 号：S723[农业科学—林木遗传育种]