氮掺杂木质素基碳量子点在次氯酸根离子检测中的应用  

Nitrogen Doped Lignin-based Carbon Quantum Dots for Detection of Hypochlorite Ion

作　　者：张晓君 武佳龙 乔楠[3] 于大禹[1] 孙墨杰[1] 陈景[2] ZHANG Xiaojun;WU Jialong;QIAO Nan;YU Dayu;SUN Mojie;CHEN Jing(School of Chemical Engineering,Northeast Electric Power University,Jilin 132012,Jilin,China;Ningbo Institute of Materials Technology&Engineering,Ningbo 315201,Zhejiang,China;School of Civil Engineering and Architecture,Northeast Electric Power University,Jilin 132012,Jilin,China)

机构地区：[1]东北电力大学化学工程学院,吉林吉林132012 [2]中国科学院宁波材料技术与工程研究所,浙江宁波315201 [3]东北电力大学建筑工程学院,吉林吉林132012

出　　处：《材料导报》2024年第24期84-88,共5页Materials Reports

基　　金：吉林省科技发展计划项目(20190902014TC,20210201058GX)。

摘　　要：采用三聚氰胺提供氮源,对碳量子点(CQDs)进行氮掺杂,利用一步水热法制备氮掺杂木质素基碳量子点(N-CQDs)。通过氮掺杂形成共轭体系并引入氨基,达到提高其荧光强度的目的。对N-CQDs进行表征和测试,结果表明N-CQDs的平均粒径为2.7 nm,由许多官能团构成,如氨基、羟基、羧基、醚键、碳氮单键、碳氮双键、以及苯环结构等。其最佳激发、发射波长分别为400 nm、500 nm,紫外可见光谱中位于260 nm处的吸收峰是酶解木质素的C=O键与苯环结构的π-π*共轭吸收峰和来自三聚氰胺C=N键的π-π*共轭吸收峰,在315 nm处的吸收峰归因于酶解木质素C=O键或苯环的n-π*跃迁,以及三聚氰胺的C-N键和C=N键的n-π*跃迁。将N-CQDs应用于离子检测,其对次氯酸根(ClO^(-))有猝灭作用,对ClO^(-)具有特异性检测,检测限为1.21μmol/L。N-CQDs可以成功进入活细胞,对活体细胞无害,并能观察到细胞质中的亮绿色荧光。N-CQDs具有良好的次氯酸根离子检测效果和荧光效应,且对细胞无毒性,在离子检测和医疗方面具有一定的应用前景。Using melamine as a nitrogen source,carbon quantum dots(CQDs)were doped with nitrogen.Nitrogen doped lignin-based carbon quantum dots(N-CQDs)were prepared through a one-step hydrothermal method.Doping nitrogen formed conjugation systems,introduced amino groups,thus improved the fluorescence intensity.Characterization and test of N-CQDs were performed.The average particle size of N-CQDs was 2.7 nm.N-CQDs were composed of many functional groups,including amino,hydroxyl,carboxyl,ether bonds,single bonds between carbon and nitrogen,double bonds between carbon and nitrogen,and benzene ring structures.The optimum excitation and emission wavelength were 400 nm and 500 nm respectively.In the UV-Vis absorption spectra,the absorption peak at 260 nm was due toπ-π*leaps of C=O bond in enzymatic hydrolysis lignin and benzene ring,π-π*leaps of C=N bond in melamine.The absorption peak at 315 nm was due to n-π*leaps of C=O bond in enzymatic hydrolysis lignin or benzene ring,n-π*leaps of C-N bond and C=N bond in melamine.N-CQDs were applied to anion detection,having a sudden inactivation effect on hypochlorite ion(ClO^(-)).N-CQDs had specific detection for ClO^(-) and the detection limits were 1.21μmol/L.N-CQDs could enter live cells successfully and N-CQDs,is harmless to cells,and bright green fluorescence in the cytoplasm of cells could be observed.Based on the good detection for ClO^(-),fluorescence effect and harmlessness to cells,N-CQDs have some potential applications in the ion detection and medical field.

关 键 词：碳量子点 氮掺杂 ClO^(-)检测 木质素 细胞 

分 类 号：O69[理学—化学]